Outdoor photoacclimation of two Chlorella strains characterized by normal and reduced light-harvesting antennas: photosynthetic activity and chlorophyll-protein organization

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F22%3A00561863" target="_blank" >RIV/61388971:_____/22:00561863 - isvavai.cz</a>

Alternative codes found

RIV/60076658:12310/22:43904948

Result on the web

<a href="https://link.springer.com/article/10.1007/s10811-022-02803-1" target="_blank" >https://link.springer.com/article/10.1007/s10811-022-02803-1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10811-022-02803-1" target="_blank" >10.1007/s10811-022-02803-1</a>

Result language

angličtina

Original language name

Outdoor photoacclimation of two Chlorella strains characterized by normal and reduced light-harvesting antennas: photosynthetic activity and chlorophyll-protein organization

Original language description

Photoacclimation of two Chlorella cultures strain g-120 characterised by a reduced size of light-harvesting antenna complex (LHC) and strain R-117 with full antenna size was studied during 5-day outdoor trials. The aim was to correlate the functional and structural changes in the photosynthetic apparatus to culture growth, photochemical activity and thylakoid composition of chlorophyll (Chl)-protein complexes and corresponding polypeptides. Chlorella g-120 was characterized by a low Chl/biomass ratio (< 0.5% of dry weight), about four times lower compared to Chlorella R-117. The important observation was that the high molecular mass Chl-binding protein supercomplexes, i.e. Photosystem II (PSII) and Photosystem I (PSI) cores associated with LHCs were physically missing or negligible in Chlorella g-120. However, there were no visible changes in Chl-protein composition in the g-120 strain during its acclimation to phototrophic conditions. Measurement of the effective absorption cross-section of PSII centres confirmed a markedly reduced functional antenna size in Chlorella g-120 as compared to R-117 which coincided with the absence of the PSII-LHC supercomplexes. We demonstrated that Chlorella g-120 represents a typical reduced antenna-size strain due to its Chl-protein composition. As compared to the full-antenna Chlorella R-117 strain, the outdoor cultures of Chlorella g-120 showed significantly lower oxygen production and electron transport rate measured in-situ. On the contrary, Chlorella g-120 revealed increased futile energy dissipation via non-photochemical quenching and higher respiration compared to Chlorella R-117. Consequently, the potential use of microalgae strains with reduced LHCII for outdoor mass cultivation may not be as straightforward as anticipated from laboratory experiments.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/TN01000048" target="_blank" >TN01000048: Biorefining as circulation technology</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Applied Phycology

ISSN

0921-8971

e-ISSN

1573-5176

Volume of the periodical

34

Issue of the periodical within the volume

5

Country of publishing house

US - UNITED STATES

Number of pages

15

Pages from-to

2339-2353

UT code for WoS article

000829689600001

EID of the result in the Scopus database

2-s2.0-85134763624