Towards spruce-type photosystem II: Consequences of the loss of light-harvesting proteins LHCB3 and LHCB6 in Arabidopsis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F21%3A00551720" target="_blank" >RIV/61389030:_____/21:00551720 - isvavai.cz</a>
Alternative codes found
RIV/86652079:_____/21:00551720 RIV/61988987:17310/21:A2202ABS RIV/00216224:14740/21:00123926
Result on the web
<a href="http://doi.org/10.1093/plphys/kiab396" target="_blank" >http://doi.org/10.1093/plphys/kiab396</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/plphys/kiab396" target="_blank" >10.1093/plphys/kiab396</a>
Alternative languages
Result language
angličtina
Original language name
Towards spruce-type photosystem II: Consequences of the loss of light-harvesting proteins LHCB3 and LHCB6 in Arabidopsis
Original language description
The largest stable photosystem II (PSII) supercomplex in land plants (C2S2M2) consists of a core complex dimer (C2), two strongly (S2) and two moderately (M2) bound light-harvesting protein (LHCB) trimers attached to C2 via monomeric antenna proteins LHCB4-6. Recently, we have shown that LHCB3 and LHCB6, presumably essential for land plants, are missing in Norway spruce (Picea abies), which results in a unique structure of its C2S2M2 supercomplex. Here, we performed structure- function characterization of PSII supercomplexes in Arabidopsis (Arabidopsis thaliana) mutants lhcb3, lhcb6, and lhcb3 lhcb6 to examine the possibility of the formation of the spruce-type PSII supercomplex in angiosperms. Unlike in spruce, in Arabidopsis both LHCB3 and LHCB6 are necessary for stable binding of the M trimer to PSII core. The spruce-type PSII supercomplex was observed with low abundance only in the lhcb3 plants and its formation did not require the presence of LHCB4.3, the only LHCB4-type protein in spruce. Electron microscopy analysis of grana membranes revealed that the majority of PSII in lhcb6 and namely in lhcb3 lhcb6 mutants were arranged into C2S2semi-crystalline arrays, some of which appeared to structurally restrict plastoquinone diffusion. Mutants without LHCB6 were characterized by fast induction of non-photochemical quenching and, on the contrary to the previous lhcb6 study, by only transient slowdown of electron transport between PSII and PSI. We hypothesize that these functional changes, associated with the arrangement of PSII into C2S2arrays in thylakoids, may be important for the photoprotection of both PSI and PSII upon abrupt high-light exposure.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10610 - Biophysics
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Plant Physiology
ISSN
0032-0889
e-ISSN
1532-2548
Volume of the periodical
187
Issue of the periodical within the volume
4
Country of publishing house
US - UNITED STATES
Number of pages
25
Pages from-to
2691-2715
UT code for WoS article
000733403700059
EID of the result in the Scopus database
2-s2.0-85121125021