Unique organization of photosystem II supercomplexes and megacomplexes in Norway spruce

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F20%3A73604417" target="_blank" >RIV/61989592:15310/20:73604417 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14740/20:00121451

Result on the web

<a href="https://onlinelibrary.wiley.com/doi/full/10.1111/tpj.14918" target="_blank" >https://onlinelibrary.wiley.com/doi/full/10.1111/tpj.14918</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/tpj.14918" target="_blank" >10.1111/tpj.14918</a>

Result language

angličtina

Original language name

Unique organization of photosystem II supercomplexes and megacomplexes in Norway spruce

Original language description

Photosystem II (PSII) complexes are organized into large supercomplexes with variable amounts of lightharvesting proteins (Lhcb). A typical PSII supercomplex in plants is formed by four trimers of Lhcb proteins (LHCII trimers), which are bound to the PSII core dimer via monomeric antenna proteins. However, the architecture of PSII supercomplexes in Norway spruce[Picea abies (L.) Karst.] is different, most likely due to a lack of two Lhcb proteins, Lhcb6 and Lhcb3. Interestingly, the spruce PSII supercomplex shares similar structural features with its counterpart in the green alga Chlamydomonas reinhardtii [Kouril et al. (2016) New Phytol. 210, 808–814]. Here we present a single-particle electron microscopy study of isolated PSII supercomplexes from Norway spruce that revealed binding of a variable amount of LHCII trimers to the PSII core dimer at positions that have never been observed in any other plant species so far. The largest spruce PSII supercomplex, which was found to bind eight LHCII trimers, is even larger than the current largest known PSII supercomplex from C. reinhardtii. We have also shown that the spruce PSII supercomplexes can form various types of PSII megacomplexes, which were also identified in intact grana membranes. Some of these large PSII supercomplexes and megacomplexes were identified also in Pinus sylvestris, another representative of the Pinaceae family. The structural variability and complexity of LHCII organization in Pinaceae seems to be related to the absence of Lhcb6 and Lhcb3 in this family, and may be beneficial for the optimization of light-harvesting under varying environmental conditions.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10610 - Biophysics

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

PLANT JOURNAL

ISSN

0960-7412

e-ISSN

—

Volume of the periodical

2020

Issue of the periodical within the volume

104

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

215-225

UT code for WoS article

000557081500001

EID of the result in the Scopus database

2-s2.0-85088784827