Bio-production of eicosapentaenoic acid from the diatom Nanofrustulum shiloi via two-step high performance countercurrent chromatography
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F22%3A00565820" target="_blank" >RIV/61388971:_____/22:00565820 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12310/22:43905310
Result on the web
<a href="https://link.springer.com/article/10.1007/s10811-022-02816-w" target="_blank" >https://link.springer.com/article/10.1007/s10811-022-02816-w</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s10811-022-02816-w" target="_blank" >10.1007/s10811-022-02816-w</a>
Alternative languages
Result language
angličtina
Original language name
Bio-production of eicosapentaenoic acid from the diatom Nanofrustulum shiloi via two-step high performance countercurrent chromatography
Original language description
Eicosapentaenoic acid (EPA) is an essential polyunsaturated fatty acid of nutritional and physiological relevance for humans. It is commercially produced mainly from fish oil, but new sustainable sources are required. In this study, pure EPA ethyl ester was produced from Nanofrustulum shiloi biomass by integrating two separation steps via high performance countercurrent chromatography (HPCCC). The HPCCC first step consisted of a 10 separation cycles (1200 mg sample per cycle) process, each cycle consisting of three stages comprising EPA fraction separation in reversed phase elution mode (lower polar phase used as mobile phase), with stationary phase extrusion (by switching the pumping of the mobile phase to the stationary phase, while maintaining the column rotation), and hydrodynamic equilibrium achievement inside the column. The process led to 982.1 mg of a fraction rich in EPA with a purity of 71%. To improve the purity of the EPA fraction, an HPCCC second step was performed leading to 698.97 mg of EPA ethyl ester with a purity of 99%, and a recovery of 98%. To avoid unnecessary waste of solvents, liquid phases were formulated based on a priori NMR measurement. The HPCCC process throughput was 0.936 g h(-1), while the efficiency per gram of EPA was 0.054 g h(-1). Environmental risk and process evaluation factors were used for the evaluation of the separation process. Overall, this isolation approach may represent a useful model for the efficient production of pure EPA from microalgae.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Applied Phycology
ISSN
0921-8971
e-ISSN
1573-5176
Volume of the periodical
34
Issue of the periodical within the volume
SEP 1
Country of publishing house
US - UNITED STATES
Number of pages
16
Pages from-to
2995-3010
UT code for WoS article
000847965100001
EID of the result in the Scopus database
2-s2.0-85137249326