Reducing the number of accepted species in Aspergillus series Nigri
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F22%3A00567685" target="_blank" >RIV/61388971:_____/22:00567685 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/22:10457287
Result on the web
<a href="https://www.ingentaconnect.com/content/wfbi/sim/2022/00000102/00000001/art00004#" target="_blank" >https://www.ingentaconnect.com/content/wfbi/sim/2022/00000102/00000001/art00004#</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3114/sim.2022.102.03" target="_blank" >10.3114/sim.2022.102.03</a>
Alternative languages
Result language
angličtina
Original language name
Reducing the number of accepted species in Aspergillus series Nigri
Original language description
The Aspergillus series Nigri contains biotechnologically and medically important species. They can produce hazardous mycotoxins, which is relevant due to the frequent occurrence of these species on foodstuffs and in the indoor environment. The taxonomy of the series has undergone numerous rearrangements, and currently, there are 14 species accepted in the series, most of which are considered cryptic. Species-level identifications are, however, problematic or impossible for many isolates even when using DNA sequencing or MALDI-TOF mass spectrometry, indicating a possible problem in the definition of species limits or the presence of undescribed species diversity. To re-examine the species boundaries, we collected DNA sequences from three phylogenetic markers (benA, CaM and RPB2) for 276 strains from series Nigri and generated 18 new whole-genome sequences. With the threegene dataset, we employed phylogenetic methods based on the multispecies coalescence model, including four single-locus methods (GMYC, bGMYC, PTP and bPTP) and one multilocus method (STACEY). From a total of 15 methods and their various settings, 11 supported the recognition of only three species corresponding to the three main phylogenetic lineages: A. niger, A. tubingensis and A. brasiliensis. Similarly, recognition of these three species was supported by the GCPSR approach (Genealogical Concordance Phylogenetic Species Recognition) and analysis in DELINEATE software. We also showed that the phylogeny based on benA, CaM and RPB2 is suboptimal and displays significant differences from a phylogeny constructed using 5 752 single-copy orthologous proteins, therefore, the results of the delimitation methods may be subject to a higher than usual level of uncertainty. To overcome this, we randomly selected 200 genes from these genomes and performed ten independent STACEY analyses, each with 20 genes.
Czech name
—
Czech description
—
Classification
Type
J<sub>ost</sub> - Miscellaneous article in a specialist periodical
CEP classification
—
OECD FORD branch
10606 - Microbiology
Result continuities
Project
<a href="/en/project/NU21-05-00681" target="_blank" >NU21-05-00681: Consequences of hybridization for spread of antifungal resistance and species delimitation in pathogenic fungi: model study in Aspergillus and Trichophyton</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Studies in Mycology
ISSN
0166-0616
e-ISSN
1872-9797
Volume of the periodical
102
Issue of the periodical within the volume
1
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
38
Pages from-to
95-132
UT code for WoS article
000968749700003
EID of the result in the Scopus database
—