Reduced phosphatidylcholine level in the intestinal mucus layer of prediabetic NOD mice

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F23%3A00572415" target="_blank" >RIV/61388971:_____/23:00572415 - isvavai.cz</a>

Alternative codes found

RIV/49777513:23520/23:43971200

Result on the web

<a href="https://onlinelibrary.wiley.com/doi/10.1111/apm.13302" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1111/apm.13302</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/apm.13302" target="_blank" >10.1111/apm.13302</a>

Result language

angličtina

Original language name

Reduced phosphatidylcholine level in the intestinal mucus layer of prediabetic NOD mice

Original language description

Type 1 diabetes (T1D) is an autoimmune disease with rising incidence. Pre- and manifest T1D is associated with intestinal barrier dysfunction, skewed microbiota composition, and serum dyslipidemia. The intestinal mucus layer protects against pathogens and its structure and phosphatidylcholine (PC) lipid composition may be compromised in T1D, potentially contributing to barrier dysfunction. This study compared prediabetic Non-Obese Diabetic (NOD) mice to healthy C57BL/6 mice by analyzing the intestinal mucus PC profile by shotgun lipidomics, plasma metabolomics by mass spectrometry and nuclear magnetic resonance, intestinal mucus production by histology, and cecal microbiota composition by 16 S rRNA sequencing. Jejunal mucus PC class levels were decreased in early prediabetic NOD vs C57BL/6 mice. In colonic mucus of NOD mice, the level of several PC species was reduced throughout prediabetes. In plasma, similar reductions of PC species were observed in early prediabetic NOD mice, where also increased beta-oxidation was prominent. No histological alterations were found in jejunal nor colonic mucus between the mouse strains. However, the b-diversity of the cecal microbiota composition differed between prediabetic NOD and C57BL/6 mice, and the bacterial species driving this difference were related to decreased short-chain fatty acid (SCFA)-production in the NOD mice. This study reports reduced levels of PCs in the intestinal mucus layer and plasma of prediabetic NOD mice as well as reduced proportions of SCFA-producing bacteria in cecal content at early prediabetes, possibly contributing to intestinal barrier dysfunction and T1D.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10606 - Microbiology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Pathologica Microbiologica et Immunologica Scandinavica

ISSN

0903-4641

e-ISSN

1600-0463

Volume of the periodical

131

Issue of the periodical within the volume

6

Country of publishing house

DK - DENMARK

Number of pages

12

Pages from-to

237-248

UT code for WoS article

000956442500001

EID of the result in the Scopus database

2-s2.0-85150463605