Top-Down Proteoform Analysis by 2D MS with Quadrupolar Detection
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F23%3A00579428" target="_blank" >RIV/61388971:_____/23:00579428 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/23:10471480
Result on the web
<a href="https://pubs.acs.org/doi/10.1021/acs.analchem.3c02225" target="_blank" >https://pubs.acs.org/doi/10.1021/acs.analchem.3c02225</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acs.analchem.3c02225" target="_blank" >10.1021/acs.analchem.3c02225</a>
Alternative languages
Result language
angličtina
Original language name
Top-Down Proteoform Analysis by 2D MS with Quadrupolar Detection
Original language description
Two-dimensional mass spectrometry (2D MS) is a multiplexed tandem mass spectrometry method that does not rely on ion isolation to correlate the precursor and fragment ions. On a Fourier transform ion cyclotron resonance mass spectrometer (FT-ICR MS), 2D MS instead uses the modulation of precursor ion radii inside the ICR cell before fragmentation and yields 2D mass spectra that show the fragmentation patterns of all the analytes. In this study, we perform 2D MS for the first time with quadrupolar detection in a dynamically harmonized ICR cell. We discuss the advantages of quadrupolar detection in 2D MS and how we adapted existing data processing techniques for accurate frequency-to-mass conversion. We apply 2D MS with quadrupolar detection to the top-down analysis of covalently labeled ubiquitin with ECD fragmentation, and we develop a workflow for label-free relative quantification of biomolecule isoforms in 2D MS.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytical Chemistry
ISSN
0003-2700
e-ISSN
1520-6882
Volume of the periodical
95
Issue of the periodical within the volume
44
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
16123-16130
UT code for WoS article
001101047900001
EID of the result in the Scopus database
2-s2.0-85178259308