Structural characterization of two prototypical repressors of SorC family reveals tetrameric assemblies on DNA and mechanism of function
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F24%3A00586786" target="_blank" >RIV/61388971:_____/24:00586786 - isvavai.cz</a>
Alternative codes found
RIV/61388963:_____/24:00586786 RIV/00216224:14740/24:00139056
Result on the web
<a href="https://doi.org/10.1093/nar/gkae434" target="_blank" >https://doi.org/10.1093/nar/gkae434</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/nar/gkae434" target="_blank" >10.1093/nar/gkae434</a>
Alternative languages
Result language
angličtina
Original language name
Structural characterization of two prototypical repressors of SorC family reveals tetrameric assemblies on DNA and mechanism of function
Original language description
The SorC family of transcriptional regulators plays a crucial role in controlling the carbohydrate metabolism and quorum sensing. We employed an integrative approach combining X-ray crystallography and cryo-electron microscopy to investigate architecture and functional mechanism of two prototypical representatives of two sub-classes of the SorC family: DeoR and CggR from Bacillus subtilis. Despite possessing distinct DNA-binding domains, both proteins form similar tetrameric assemblies when bound to their respective DNA operators. Structural analysis elucidates the process by which the CggR-regulated gapA operon is derepressed through the action of two effectors: fructose-1,6-bisphosphate and newly confirmed dihydroxyacetone phosphate. Our findings provide the first comprehensive understanding of the DNA binding mechanism of the SorC-family proteins, shedding new light on their functional characteristics.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Nucleic Acids Research
ISSN
0305-1048
e-ISSN
1362-4962
Volume of the periodical
52
Issue of the periodical within the volume
12
Country of publishing house
US - UNITED STATES
Number of pages
16
Pages from-to
7305-7320
UT code for WoS article
001241820300001
EID of the result in the Scopus database
2-s2.0-85197970745