ABNOH-Linked Nucleotides and DNA for Bioconjugation and Cross-linking with Tryptophan-Containing Peptides and Proteins
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F24%3A00587190" target="_blank" >RIV/61388971:_____/24:00587190 - isvavai.cz</a>
Alternative codes found
RIV/61388963:_____/24:00587190 RIV/00216208:11310/24:10495136
Result on the web
<a href="https://doi.org/10.1002/chem.202402151" target="_blank" >https://doi.org/10.1002/chem.202402151</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/chem.202402151" target="_blank" >10.1002/chem.202402151</a>
Alternative languages
Result language
angličtina
Original language name
ABNOH-Linked Nucleotides and DNA for Bioconjugation and Cross-linking with Tryptophan-Containing Peptides and Proteins
Original language description
Reactive N-hydroxy-9-azabicyclo[3.3.1]nonane (ABNOH) linked 2’-deoxyuridine 5’-O-mono- and triphosphates were synthesized through a CuAAC click reaction of ABNO-H-PEG4-N3 with 5-ethynyl-dUMP or -dUTP. The modified triphosphate was used as substrate for enzymatic synthesis of modified DNA probes with KOD XL DNA polymerase. The keto-ABNO radical reacted with tryptophan (Trp) and Trp-containing peptides to form a stable 3-fused hexahydropyrrolo-indole conjugates. Similarly modified ABNO-H-linked nucleotides reacted with Trp-containing peptides to form a stable conjugate in the presence but surprisingly even in the absence of NaNO2 (presumably through activation by O2). The reactive ABNO-H-modified DNA probe was used for bioconjugations and crosslinking with Trp-containing peptides or proteins.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Chemistry - A European Journal
ISSN
0947-6539
e-ISSN
1521-3765
Volume of the periodical
30
Issue of the periodical within the volume
49
Country of publishing house
DE - GERMANY
Number of pages
9
Pages from-to
e202402151
UT code for WoS article
001289658400001
EID of the result in the Scopus database
2-s2.0-85200955670