Aldoxime dehydratases: production, immobilization, and use in multistep processes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F24%3A00601349" target="_blank" >RIV/61388971:_____/24:00601349 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/24:10495725

Result on the web

<a href="https://link.springer.com/article/10.1007/s00253-024-13272-6" target="_blank" >https://link.springer.com/article/10.1007/s00253-024-13272-6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00253-024-13272-6" target="_blank" >10.1007/s00253-024-13272-6</a>

Result language

angličtina

Original language name

Aldoxime dehydratases: production, immobilization, and use in multistep processes

Original language description

The synthesis of nitriles is of utmost importance for preparative organic chemistry. The classical routes are often associated with disadvantages such as toxicity of the reagents and drastic conditions. The uses of enzymes like aldoxime dehydratases (Oxds) and hydroxynitrile lyases constitute attractive benign alternatives. In this review, we summarize the recent trends regarding Oxds. Thousands of oxd genes were sequenced but less than thirty Oxds were investigated on protein level. We give an overview of these Oxds, their sequence analysis, conditions required for their overexpression, and their purification and assays. We then focus on the use of Oxds especially in multistep reactions combining the chemical or chemoenzymatic synthesis of aldoximes from different starting materials with the enzymatic dehydration of aldoximes to nitriles, possibly followed by the hydration of nitriles to amides. Progress in Oxd immobilization is also highlighted. Based on data published mainly in the last 5years, we evaluate the industrial prospects of these enzyme processes in comparison with some other innovations in nitrile synthesis. KEY POINTS: Aldoxime dehydratases (Oxds) are promising for cyanide-free routes to nitriles A comprehensive overview of wet-lab explored Oxds is provided Recent trends include combining Oxds with other enzymes or chemical catalysts.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Applied Microbiology and Biotechnology

ISSN

0175-7598

e-ISSN

1432-0614

Volume of the periodical

108

Issue of the periodical within the volume

1

Country of publishing house

DE - GERMANY

Number of pages

19

Pages from-to

518

UT code for WoS article

001355204400001

EID of the result in the Scopus database

2-s2.0-85209173862