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Dual thermo- and pH-responsive polymer nanoparticle assemblies for potential stimuli-controlled drug delivery

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F25%3A00604832" target="_blank" >RIV/61388971:_____/25:00604832 - isvavai.cz</a>

  • Alternative codes found

    RIV/61389013:_____/25:00604832

  • Result on the web

    <a href="https://pubs.acs.org/doi/10.1021/acsabm.4c01167" target="_blank" >https://pubs.acs.org/doi/10.1021/acsabm.4c01167</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1021/acsabm.4c01167" target="_blank" >10.1021/acsabm.4c01167</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Dual thermo- and pH-responsive polymer nanoparticle assemblies for potential stimuli-controlled drug delivery

  • Original language description

    The development of stimuli-responsive drug delivery systems enables targeted delivery and environment-controlled drug release, thereby minimizing off-target effects and systemic toxicity. We prepared and studied tailor-made dual-responsive systems (thermo- and pH-) based on synthetic diblock copolymers consisting of a fully hydrophilic block of poly[N-(1,3-dihydroxypropyl)methacrylamide] (poly(DHPMA)) and a thermoresponsive block of poly[N-(2,2-dimethyl-1,3-dioxan-5-yl)methacrylamide] (poly(DHPMA-acetal)) as drug delivery and smart stimuli-responsive materials. The copolymers were designed for eventual medical application to be fully soluble in aqueous solutions at 25 °C. However, they form well-defined nanoparticles with hydrodynamic diameters of 50–800 nm when heated above the transition temperature of 27–31 °C. This temperature range is carefully tailored to align with the human body’s physiological conditions. The formation of the nanoparticles and their subsequent decomposition was studied using dynamic light scattering (DLS), transmission electron microscopy (TEM), isothermal titration calorimetry (ITC), and nuclear magnetic resonance (NMR). 1H NMR studies confirmed that after approximately 20 h of incubation at pH 5, which closely mimics tumor microenvironment, approximately 40% of the acetal groups were hydrolyzed, and the thermoresponsive behavior of the copolymers was lost. This smart polymer response led to disintegration of the supramolecular structures, possibly releasing the therapeutic cargo. By tuning the transition temperature to the values relevant for medical applications, we ensure precise and effective drug release. In addition, our systems did not exhibit any cytotoxicity against any of the three cell lines. Our findings underscore the immense potential of these nanoparticles as eventual advanced drug delivery systems, especially for cancer therapy.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    30104 - Pharmacology and pharmacy

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2025

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    ACS Applied Bio Materials

  • ISSN

    2576-6422

  • e-ISSN

    2576-6422

  • Volume of the periodical

    8

  • Issue of the periodical within the volume

    1

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    14

  • Pages from-to

    271-284

  • UT code for WoS article

    001375023100001

  • EID of the result in the Scopus database

    2-s2.0-85211621102