Recovery of β-Carotene from Microalga Dunaliella sp. by HPCCC
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F25%3A00637066" target="_blank" >RIV/61388971:_____/25:00637066 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12310/25:43909935
Result on the web
<a href="https://www.mdpi.com/2227-9717/13/6/1812" target="_blank" >https://www.mdpi.com/2227-9717/13/6/1812</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/pr13061812" target="_blank" >10.3390/pr13061812</a>
Alternative languages
Result language
angličtina
Original language name
Recovery of β-Carotene from Microalga Dunaliella sp. by HPCCC
Original language description
beta-carotene, a high-value carotenoid widely used in the food, pharmaceutical, and cosmetics industries, is naturally synthesized by the microalga Dunaliella sp. However, the efficient extraction and purification of beta-carotene from microalgae biomass remain a technical challenge. This study presents the development of a scalable and efficient isolation method employing high-performance countercurrent chromatography (HPCCC) to recover beta-carotene from Dunaliella sp. The separation process was optimized by integrating two elution strategies (reverse phase and extrusion) using a biphasic solvent system of n-heptane and methanol (1:1, v/v). The upper phase served as the stationary phase, while the lower phase was used as the mobile phase. Two consecutive injections of 800 mg of microalgal extract each resulted in the isolation of 225.4 mg of beta-carotene with a purity of 97% and a recovery of 98%. The developed HPCCC approach represents an efficient method for beta-carotene purification and serves as a promising model for future scale-up in microalgae-based production platforms.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
20401 - Chemical engineering (plants, products)
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Processes
ISSN
2227-9717
e-ISSN
2227-9717
Volume of the periodical
13
Issue of the periodical within the volume
6
Country of publishing house
CH - SWITZERLAND
Number of pages
15
Pages from-to
1812
UT code for WoS article
001515002300001
EID of the result in the Scopus database
2-s2.0-105009298342