Global analysis of the Hfq-mediated RNA interactome discovers a MicA homolog that affects the cytotoxicity, biofilm formation, and resistance to complement of Bordetella pertussis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F25%3A00637262" target="_blank" >RIV/61388971:_____/25:00637262 - isvavai.cz</a>

Result on the web

<a href="https://academic.oup.com/nar/article/53/13/gkaf614/8192818?searchresult=1" target="_blank" >https://academic.oup.com/nar/article/53/13/gkaf614/8192818?searchresult=1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/nar/gkaf614" target="_blank" >10.1093/nar/gkaf614</a>

Result language

angličtina

Original language name

Global analysis of the Hfq-mediated RNA interactome discovers a MicA homolog that affects the cytotoxicity, biofilm formation, and resistance to complement of Bordetella pertussis

Original language description

Bordetella pertussis is a Gram-negative, strictly human re-emerging respiratory pathogen and the causative agent of whooping cough. The requirement of the RNA chaperone Hfq for the virulence of B. pertussis suggests that Hfq-dependent small regulatory RNAs (sRNAs) are involved in the virulence of this pathogen. To identify their potential mRNA targets, we applied a method combining experimental and computational approaches called RIL-seq. The majority of putative mRNA targets, including several virulence factors, interact with two sRNAs, CT_433 and CT_521, suggesting that these sRNAs may represent central riboregulatory nodes of B. pertussis. Furthermore, our data suggest that CT_532 sRNA can base pair with the 5′UTR region of ompA mRNA encoding outer membrane protein BP0943 (OmpA) and that CT_532, RNase III and Hfq are involved in the control of ompA expression. The CT_532 sRNA shares 60% identity with the E. coli sRNA MicA and its expression is also modulated by Hfq and stress conditions such as heat and cold shocks. Overall, these results suggest that CT_532 represents a MicA homolog. Importantly, the mutant lacking the first 22 nucleotides of CT_532 exhibits reduced cytotoxicity towards human macrophages and impaired biofilm production but increased resistance to complement compared to the wild type strain.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10606 - Microbiology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nucleic Acids Research

ISSN

0305-1048

e-ISSN

1362-4962

Volume of the periodical

53

Issue of the periodical within the volume

13

Country of publishing house

US - UNITED STATES

Number of pages

19

Pages from-to

gkaf614

UT code for WoS article

001523917200001

EID of the result in the Scopus database

2-s2.0-105010440140