DigDig

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F25%3A00638573" target="_blank" >RIV/61388971:_____/25:00638573 - isvavai.cz</a>
Result on the web
<a href="https://peterslab.org/DigDig/doc/" target="_blank" >https://peterslab.org/DigDig/doc/</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
DigDig
Original language description
Anotace v jazyce prácenProteolysis is a crucial step in both bottom-up and structural proteomics workflows, directly influencing peptide identification and sequence coverage in mass spectrometry-based analyses. While classical proteomics typically relies on highly specific enzymes with well-defined cleavage patterns, structural MS approaches such as hydrogen/deuterium exchange mass spectrometry (HDX-MS) often employ non-specific or semi-specific proteases, producing complex peptide mixtures that require more detailed digestion analysis. To address these needs and streamline the entire process, we developed DigDig, a standalone, Java-based software tool for evaluating and comparing proteolytic digestion across diverse experimental conditions. DigDig processes output files from common search engines and provides customizable visualizations of key digestion metrics, including sequence coverage, reproducibility, peptide redundancy, cleavage site preferences, and peptide length distributions. A distinguishing feature is its ability to detect and report repetitive peptide sequences, which are frequently missed by standard tools. We demonstrate its capabilities using datasets from both specific and non-specific digestions, highlighting its utility in digestion quality control, protease characterization, and method development, particularly in HDX-MS workflows.n
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Internal product ID
DigDig2025
Technical parameters
Samostatný program sestavený v jazyce Java fungující na běžných výpočetních strojích pod systémy Unix/Linux, Windows a MAC OS
Economical parameters
Detailní analýza LC-MS/MS s důrazem na parametrizaci proteolytického štěpení a extrakci štěpných preferencí
Owner IČO
61388971
Owner name
Mikrobiologický ústav AV ČR, v. v. i.

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