Cellular Delivery of Functional AntimiR Conjugated to Bio-Produced Gold Nanoparticles

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F25%3A00640831" target="_blank" >RIV/61388971:_____/25:00640831 - isvavai.cz</a>

Alternative codes found

RIV/46747885:24530/25:00014033

Result on the web

<a href="https://www.mdpi.com/2311-553X/11/5/66" target="_blank" >https://www.mdpi.com/2311-553X/11/5/66</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/ncrna11050066" target="_blank" >10.3390/ncrna11050066</a>

Result language

angličtina

Original language name

Cellular Delivery of Functional AntimiR Conjugated to Bio-Produced Gold Nanoparticles

Original language description

Background/Objectives: Bio-produced gold nanoparticles (AuNPs) are effective carriers of short RNAs into specialized mammalian cells. Their potential application is still limited by scarce knowledge on their uptake and intracellular fate. Gold nanoparticles that are not biologically produced (NB-AuNPs) enter specialized cells primarily via clathrin-dependent endocytosis. Unlike the NB-AuNPs, the bio AuNPs possess natural surface coatings that significantly alter the AuNPs properties. Our research aimed to reveal the cellular uptake of the AuNPs with respect to delivering a functional RNA cargo. Methods: The AuNPs were conjugated with short inhibitory RNA specific to miR 135b. Mammary cancer cells 4T1 were pretreated with inhibitors of caveolin- and clathrin-mediated endocytosis and macropinocytosis. AuNPs' uptake, fate, and miR 135b knock-down were assessed with TEM and qPCR. Results: The AuNPs-antimiR 135b conjugates entered 4T1 cells via all the tested pathways and could be seen inside the cells in early and late endosomes as well as cytoplasm. In contrast to the clathrin-dependent pathway, the caveolae-mediated endocytosis and the macropinocytosis of the AuNPs resulted in the effective targeting and reduction of the miR 135b. Conclusions: The bio-produced AuNPs can effectively enter mammalian cells simultaneously by different endocytic pathways but the delivery of functional cargo is not achieved via the clathrin-dependent endocytosis.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/EH22_010%2F0002357" target="_blank" >EH22_010/0002357: MSCA Fellowships CZ ((Institute of Microbiology of the CAS, v. v. i.)</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Non-coding RNA

ISSN

2311-553X

e-ISSN

2311-553X

Volume of the periodical

11

Issue of the periodical within the volume

5

Country of publishing house

CH - SWITZERLAND

Number of pages

22

Pages from-to

66

UT code for WoS article

001601948200001

EID of the result in the Scopus database

2-s2.0-105020179191