Two-Step Mechanism of Cellular Uptake of Cationic Gold Nanoparticles Modified by (16-Mercaptohexadecyl)trimethylammonium Bromide

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F16%3A00469671" target="_blank" >RIV/68378050:_____/16:00469671 - isvavai.cz</a>

Alternative codes found

RIV/61388971:_____/16:00469671 RIV/61389013:_____/16:00469671 RIV/00179906:_____/16:10330159 RIV/68407700:21340/16:00301061 RIV/62690094:18470/16:50005133

Result on the web

<a href="http://dx.doi.org/10.1021/acs.bioconjchem.6b00491" target="_blank" >http://dx.doi.org/10.1021/acs.bioconjchem.6b00491</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acs.bioconjchem.6b00491" target="_blank" >10.1021/acs.bioconjchem.6b00491</a>

Result language

angličtina

Original language name

Two-Step Mechanism of Cellular Uptake of Cationic Gold Nanoparticles Modified by (16-Mercaptohexadecyl)trimethylammonium Bromide

Original language description

Cationic colloidal gold nanorods (GNRs) have a great potential as a theranostic tool for diverse medical applications. GNRs' properties such as cellular internalization and stability are determined by physicochemical characteristics of their surface coating. GNRs modified by (16-mercaptohexadecyl)trimethylammonium bromide (MTAB), (MTAB)GNRs, show excellent cellular uptake. Despite their promise for biomedicine, however, relatively little is known about the cellular pathways that facilitate the uptake of GNRs, their subcellular fate and intracellular persistence. Here we studied the mechanism of cellular internalization and long-term fate of GNRs coated with MTAB, for which the synthesis was optimized to give higher yield, in various human cell types including normal diploid versus cancerous, and dividing versus nondividing (senescent) cells. The process of (MTAB)GNRs internalization into their final destination in lysosomes proceeds in two steps: (1) fast passive adhesion to cell membrane mediated by sulfated proteoglycans occurring within minutes and (2) slower active transmembrane and intracellular transport of individual nanorods via clathrin-mediated endocytosis and of aggregated nanorods via macropinocytosis. The expression of sulfated proteoglycans was the major factor determining the extent of uptake by the respective cell types. Upon uptake into proliferating cells, (MTAB)GNRs were diluted equally and relatively rapidly into daughter cells; however, in nondividing/senescent cells the loss of (MTAB)GNRs was gradual and very modest, attributable mainly to exocytosis. Exocytosed (MTAB)GNRs can again be internalized. These findings broaden our knowledge about cellular uptake of gold nanorods, a crucial prerequisite for future successful engineering of nanoparticles for biomedical applications such as photothermal cancer therapy or elimination of senescent cells as part of the emerging rejuvenation approach.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

—

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Bioconjugate Chemistry

ISSN

1043-1802

e-ISSN

—

Volume of the periodical

27

Issue of the periodical within the volume

10

Country of publishing house

US - UNITED STATES

Number of pages

17

Pages from-to

2558-2574

UT code for WoS article

000385992000039

EID of the result in the Scopus database

2-s2.0-84992397142