Polymeric IgA with unique glycans protects against necrotoxigenic E. coli O55 infection in an animal model
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F25%3A00643754" target="_blank" >RIV/61388971:_____/25:00643754 - isvavai.cz</a>
Result on the web
<a href="https://academic.oup.com/jimmunol/article/215/2/vkaf300/8317948?login=true" target="_blank" >https://academic.oup.com/jimmunol/article/215/2/vkaf300/8317948?login=true</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/jimmun/vkaf300" target="_blank" >10.1093/jimmun/vkaf300</a>
Alternative languages
Result language
angličtina
Original language name
Polymeric IgA with unique glycans protects against necrotoxigenic E. coli O55 infection in an animal model
Original language description
Mucosal immunoglobulin A (IgA) promotes the survival of commensal bacteria while it inhibits the invasion by pathogens. Bacterial coating may be mediated by antigen-specific IgA recognition, polyreactivity, and/or by the IgA-associated glycans. We compared human polyclonal secretory SIgA both in vitro and in vivo with polymeric (p) monoclonal myeloma IgA proteins of defined glycan structures to assess their protective activity against necrotoxigenic Escherichia coli O55. Specifically, we evaluated the adhesion and penetration of E. coli O55 into porcine intestinal IPEC-1 cells following preincubation of the bacteria with various pIgA1 or pIgA2 preparations. The preparation designated pIgA2(F2), which exhibited a unique N-glycan composition and demonstrated the highest level of protection in vitro, was further tested in vivo in an experimental intestinal infection model using antibody-free newborn piglets. In brief, pIgA2(F2) effectively reduced inflammatory activation of gut tissue and prevented pathological alterations in intestinal architecture, performing equally well as concurrently tested milk/colostrum-derived SIgA. Future studies would lead to the identification of the specific pIgA2-associated glycans responsible for mediating protection against targeted bacterial gut infections.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
30102 - Immunology
Result continuities
Project
<a href="/en/project/NU22-01-00077" target="_blank" >NU22-01-00077: Dynamic parameters of glucose control in relation to biomarkers in serum and intraocular fluid in diabetic patients with ocular complications</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Immunology
ISSN
0022-1767
e-ISSN
1550-6606
Volume of the periodical
215
Issue of the periodical within the volume
2
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
vkaf300
UT code for WoS article
001610189500001
EID of the result in the Scopus database
2-s2.0-105030994423