Characterisation of a setup for mixed beam exposures of cells to Am-241 alpha particles and X-rays
Result description
Exposure of humans to mixed fields of high- and low-linear energy transfer (LET) radiation occurs in many situations. From the perspective of health risk associated with exposure to mixed fields, it is important to understand the interactions between different radiation types. In most cellular investigations on mixed beams, two types of irradiations have been applied sequentially. Simultaneous irradiation is the desirable scenario but requires a dedicated irradiation facility. The authors have constructed a facility where cells can be simultaneously exposed to Am-241 alpha particles and 190-kV X-rays at 37C. This study presents the technical details and the dosimetry of the setup, as well as validates the performance of the setup for clonogenic survival in AA8 Chinese hamster ovary cells. No significant synergistic effect was observed. The relative biological effectiveness of the alpha particles was 2.56 for 37 and 1.90 for 10 clonogenic survival.
Keywords
chinese-hamster cellsperipheral-blood lymphocyteslinear-energy-transfer
The result's identifiers
Result code in IS VaVaI
Result on the web
DOI - Digital Object Identifier
Alternative languages
Result language
angličtina
Original language name
Characterisation of a setup for mixed beam exposures of cells to Am-241 alpha particles and X-rays
Original language description
Exposure of humans to mixed fields of high- and low-linear energy transfer (LET) radiation occurs in many situations. From the perspective of health risk associated with exposure to mixed fields, it is important to understand the interactions between different radiation types. In most cellular investigations on mixed beams, two types of irradiations have been applied sequentially. Simultaneous irradiation is the desirable scenario but requires a dedicated irradiation facility. The authors have constructed a facility where cells can be simultaneously exposed to Am-241 alpha particles and 190-kV X-rays at 37C. This study presents the technical details and the dosimetry of the setup, as well as validates the performance of the setup for clonogenic survival in AA8 Chinese hamster ovary cells. No significant synergistic effect was observed. The relative biological effectiveness of the alpha particles was 2.56 for 37 and 1.90 for 10 clonogenic survival.
Czech name
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Czech description
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Classification
Type
Jx - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BG - Nuclear, atomic and molecular physics, accelerators
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Radiation Protection Dosimetry
ISSN
0144-8420
e-ISSN
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Volume of the periodical
151
Issue of the periodical within the volume
3
Country of publishing house
GB - UNITED KINGDOM
Number of pages
10
Pages from-to
570-579
UT code for WoS article
000308960900023
EID of the result in the Scopus database
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Result type
Jx - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP
BG - Nuclear, atomic and molecular physics, accelerators
Year of implementation
2012