Different cadmium tolerance of two isolates of Hebeloma mesophaeum showing different basal expression levels of metallothionein (HmMT3) gene
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389005%3A_____%2F19%3A00503681" target="_blank" >RIV/61389005:_____/19:00503681 - isvavai.cz</a>
Alternative codes found
RIV/67985831:_____/19:00504081 RIV/60461373:22330/19:43919770
Result on the web
<a href="https://doi.org/10.1016/j.funbio.2018.12.006" target="_blank" >https://doi.org/10.1016/j.funbio.2018.12.006</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.funbio.2018.12.006" target="_blank" >10.1016/j.funbio.2018.12.006</a>
Alternative languages
Result language
angličtina
Original language name
Different cadmium tolerance of two isolates of Hebeloma mesophaeum showing different basal expression levels of metallothionein (HmMT3) gene
Original language description
Hebeloma mesophaeum is an ectomycorrhizal fungus frequently associated with metal disturbed environments. In this work, we examined Ag, Cd, and Zn tolerance of H. mesophaeum isolates from heavy metal-polluted (isolate Prib) and clean (isolate Rez) sites. Both mycelia showed essentially the same level of Ag and Zn tolerance, but Prib was more Cd tolerant. In short-term exposures, Prib accumulated slightly less Cd than Rez. Size exclusion chromatography of cell-free extracts and fluorescence microscopy of hyphae with a Cd-specific fluorescent tracer revealed that substantial proportion of Cd was contained in the vacuoles in both isolates. Considering that the proportion of Cd associated with fractions attributable to Cd complexes with cytosolic, metallothionein (MT) peptides was higher in Prib, we examined the copy number and basal levels of HmMTs genes in Rez and Prib. While no difference between the isolates was observed in the gene copy numbers and basal levels of HmMT1 transcripts, the basal transcription of HmMT3 was 3-fold higher in Prib. These observations suggest that MTs provide in Prib better protection against Cd. Furthermore, the higher Cd tolerance in Prib can be to some extent also supported by the efflux or reduced uptake of Cd in the hyphae.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/GA16-15065S" target="_blank" >GA16-15065S: Factors affecting heavy metal accumulation in macrofungi</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Fungal Biology
ISSN
1878-6146
e-ISSN
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Volume of the periodical
123
Issue of the periodical within the volume
3
Country of publishing house
GB - UNITED KINGDOM
Number of pages
8
Pages from-to
247-254
UT code for WoS article
000461403100008
EID of the result in the Scopus database
2-s2.0-85059540159