In Vivo Validation of Alternative FDXR Transcripts in Human Blood in Response to Ionizing Radiation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389005%3A_____%2F20%3A00536551" target="_blank" >RIV/61389005:_____/20:00536551 - isvavai.cz</a>

Alternative codes found

RIV/60162694:G44__/20:00556451

Result on the web

<a href="https://doi.org/10.3390/ijms21217851" target="_blank" >https://doi.org/10.3390/ijms21217851</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/ijms21217851" target="_blank" >10.3390/ijms21217851</a>

Result language

angličtina

Original language name

In Vivo Validation of Alternative FDXR Transcripts in Human Blood in Response to Ionizing Radiation

Original language description

Following cell stress such as ionising radiation (IR) exposure, multiple cellular pathways are activated. We recently demonstrated that ferredoxin reductase (FDXR) has a remarkable IR-induced transcriptional responsiveness in blood. Here, we provided a first comprehensive FDXR variant profile following DNA damage. First, specific quantitative real-time polymerase chain reaction (qPCR) primers were designed to establish dose-responses for eight curated FDXR variants, all up-regulated after IR in a dose-dependent manner. The potential role of gender on the expression of these variants was tested, and neither the variants response to IR nor the background level of expression was profoundly affected. Moreover, in vitro induction of inflammation temporarily counteracted IR response early after exposure. Importantly, transcriptional up-regulation of these variants was further confirmed in vivo in blood of radiotherapy patients. Full-length nanopore sequencing was performed to identify other FDXR variants and revealed the high responsiveness of FDXR-201 and FDXR-208. Moreover, FDXR-218 and FDXR-219 showed no detectable endogenous expression, but a clear detection after IR. Overall, we characterised 14 FDXR transcript variants and identified for the first time their response to DNA damage in vivo. Future studies are required to unravel the function of these splicing variants, but they already represent a new class of radiation exposure biomarkers.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10610 - Biophysics

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal of Molecular Sciences

ISSN

1661-6596

e-ISSN

—

Volume of the periodical

21

Issue of the periodical within the volume

21

Country of publishing house

CH - SWITZERLAND

Number of pages

18

Pages from-to

7851

UT code for WoS article

000589122100001

EID of the result in the Scopus database

2-s2.0-85094122103