Eda controls the size of the enamel knot during incisor development

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389005%3A_____%2F23%3A00568789" target="_blank" >RIV/61389005:_____/23:00568789 - isvavai.cz</a>

Alternative codes found

RIV/67985904:_____/23:00568789 RIV/00216208:11110/23:10454867 RIV/00216208:11310/23:10454867 RIV/00216208:11120/23:43925099 RIV/00064165:_____/23:10454867

Result on the web

<a href="https://doi.org/10.3389/fphys.2022.1033130" target="_blank" >https://doi.org/10.3389/fphys.2022.1033130</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fphys.2022.1033130" target="_blank" >10.3389/fphys.2022.1033130</a>

Result language

angličtina

Original language name

Eda controls the size of the enamel knot during incisor development

Original language description

Ectodysplasin (Eda) plays important roles in both shaping the developing tooth and establishing the number of teeth within the tooth row. Sonic hedgehog (Shh) has been shown to act downstream of Eda and is involved in the initiation of tooth development. Eda-/- mice possess hypoplastic and hypomineralized incisors and show changes in tooth number in the molar region. In the present study we used 3D reconstruction combined with expression analysis, cell lineage tracing experiments, and western blot analysis in order to investigate the formation of the incisor germs in Eda-/- mice. We show that a lack of functional Eda protein during early stages of incisor tooth germ development had minimal impact on development of the early expression of Shh in the incisor, a region proposed to mark formation of a rudimental incisor placode and act as an initiating signalling centre. In contrast, deficiency of Eda protein had a later impact on expression of Shh in the primary enamel knot of the functional tooth. Eda-/- mice had a smaller region where Shh was expressed, and a reduced contribution from Shh descendant cells. The reduction in the enamel knot led to the formation of an abnormal enamel organ creating a hypoplastic functional incisor. Eda therefore appears to influence the spatial formation of the successional signalling centres during odontogenesis.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10301 - Atomic, molecular and chemical physics (physics of atoms and molecules including collision, interaction with radiation, magnetic resonances, Mössbauer effect)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in Physiology

ISSN

1664-042X

e-ISSN

1664-042X

Volume of the periodical

13

Issue of the periodical within the volume

JAN

Country of publishing house

CH - SWITZERLAND

Number of pages

14

Pages from-to

1033130

UT code for WoS article

000921369500001

EID of the result in the Scopus database

2-s2.0-85146907985