Effect of elevated temperature and hydrocortisone addition on the proliferation of fibroblasts

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389005%3A_____%2F24%3A00586608" target="_blank" >RIV/61389005:_____/24:00586608 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11110/24:10482902 RIV/00216208:11310/24:10482902
Result on the web
<a href="https://doi.org/10.1007/s00418-024-02295-9" target="_blank" >https://doi.org/10.1007/s00418-024-02295-9</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00418-024-02295-9" target="_blank" >10.1007/s00418-024-02295-9</a>

Result language
angličtina
Original language name
Effect of elevated temperature and hydrocortisone addition on the proliferation of fibroblasts
Original language description
Hyperthermia along with hydrocortisone (HC) are proven teratogens that can negatively influence embryo development during early pregnancy. Proliferation of cells is one of the main developmental processes during the early embryogenesis. This study was focused on testing the effect of elevated temperature and HC addition on proliferation of cells in in vitro cultures. The V79-4 cell line was treated with HC and cultured in vitro at 37 degrees C or 39 degrees C, respectively. To reveal the effect of both factors, the proliferation of cells cultured under different conditions was evaluated using various approaches (colony formation assay, generation of growth curves, computation of doubling times, and mitotic index estimation). Our results indicate that a short-term exposure to elevated temperature slightly stimulates and a long-term exposure suppresses cell proliferation. However, HC (0.1 mg/ml) acts as a stimulator of cell proliferation. Interestingly, the interaction of HC and long-term elevated temperature (39 degrees C) exposure results in at least partial compensation of the negative impact of elevated temperature by HC addition and in higher proliferation if compared with cells cultured at 39 degrees C without addition of HC.
Czech name
—
Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10610 - Biophysics

Project
<a href="/en/project/EF16_019%2F0000728" target="_blank" >EF16_019/0000728: Ultra-trace isotope research in social and environmental studies using accelerator mass spectrometry</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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