Comparative analysis of pediatric SHH medulloblastoma DAOY spheres and adherent monolayers: implications for medulloblastoma research
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389005%3A_____%2F25%3A00616477" target="_blank" >RIV/61389005:_____/25:00616477 - isvavai.cz</a>
Alternative codes found
RIV/68378050:_____/25:00645083 RIV/68407700:21340/25:00380560 RIV/00216208:11310/25:10498750 RIV/00023884:_____/25:00009969
Result on the web
<a href="https://cancerci.biomedcentral.com/articles/10.1186/s12935-025-03646-9" target="_blank" >https://cancerci.biomedcentral.com/articles/10.1186/s12935-025-03646-9</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/s12935-025-03646-9" target="_blank" >10.1186/s12935-025-03646-9</a>
Alternative languages
Result language
angličtina
Original language name
Comparative analysis of pediatric SHH medulloblastoma DAOY spheres and adherent monolayers: implications for medulloblastoma research
Original language description
Medulloblastoma, the most prevalent brain tumor among children, requires a comprehensive understanding of its cellular characteristics for effective research and treatment. In this study, we focused on DAOY, a permanent cell line of medulloblastoma, and investigated the unique properties of DAOY cells when cultured as floating multicellular aggregates called spheres, as opposed to adherent monolayers. Through our comprehensive analysis, we identified distinct characteristics associated with DAOY spheres. Our findings demonstrate that DAOY spheres express markers for both neural stem cells, such as CD133 (PROM1), and differentiated neurons, exemplified by MAP2. Additionally, our investigation revealed that spheres-derived cells exhibit heightened resistance to ionizing radiation compared to adherent cells. Consequently, our results indicate that caution is advised when interpreting experimental results obtained from adherent cell cultures and extrapolating them to in vivo situations.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30224 - Radiology, nuclear medicine and medical imaging
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Cancer Cell International
ISSN
1475-2867
e-ISSN
1475-2867
Volume of the periodical
25
Issue of the periodical within the volume
1
Country of publishing house
GB - UNITED KINGDOM
Number of pages
15
Pages from-to
22
UT code for WoS article
001402126100002
EID of the result in the Scopus database
2-s2.0-85217660831