Chitinolytic activities of Clostridium sp. JM2 isolated from stool of human administered per orally by chitosan

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F08%3A00325027" target="_blank" >RIV/61389013:_____/08:00325027 - isvavai.cz</a>

Alternative codes found

RIV/67985904:_____/08:00325027

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Chitinolytic activities of Clostridium sp. JM2 isolated from stool of human administered per orally by chitosan

Original language description

The novel chitinolytic bacterium Clostridium beijerinckii strain JM2 was isolated from the stool of healthy volunteers supplied daily per orally with 3 g of chitosan. The bacterium grown on colloidal chitin produced a complete array of chitinolytic enzymes. Significant activities of endochitinase, exochitinase and chitosanase were excreted into the medium (301, 282 and 268 nkat/mu g protein, respectively). The high cellular activity of N-acetyl-beta-glucosaminidase (NAGase) and chitosanase were detected(732.4 and 154 nkat/mu g protein, respectively). NAGase activity represented the main activity associated with the cellular fraction. The activities of both enzymes tested increased from 20 to 50 C; the optimum reaction temperature estimated being 50 C.Endochitinase as well as NAGase showed an activity in the pH interval of 4.0-8.0; the optimum pH values were 6.5 and 6.0, respectively.

Czech name

Chitinolytické aktivity Clostridia sp. JM2 izolovaného z lidské stolice nahrazeného chitosanem

Czech description

Chitinolytické aktivity Clostridia sp. JM2 izolovaného z lidské stolice nahrazeného chitosanem.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

<a href="/en/project/GA525%2F05%2F2584" target="_blank" >GA525/05/2584: The effect of chitin and chitosan in the human bowel</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2008

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Folia Microbiologica

ISSN

0015-5632

e-ISSN

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Volume of the periodical

53

Issue of the periodical within the volume

3

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

6

Pages from-to

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UT code for WoS article

000257941200016

EID of the result in the Scopus database

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