Excretome of the chitinolytic bacterium Clostridium paraputrificum J4

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F12%3A00387248" target="_blank" >RIV/61389013:_____/12:00387248 - isvavai.cz</a>

Alternative codes found

RIV/67985904:_____/12:00387248

Result on the web

<a href="http://dx.doi.org/10.1007/s12223-012-0137-2" target="_blank" >http://dx.doi.org/10.1007/s12223-012-0137-2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12223-012-0137-2" target="_blank" >10.1007/s12223-012-0137-2</a>

Result language

angličtina

Original language name

Excretome of the chitinolytic bacterium Clostridium paraputrificum J4

Original language description

A strictly anaerobic mesophilic chitinolytic bacterial strain identified as Clostridium paraputrificum J4 was isolated from human feces. In response to various types of growth substrates, the bacterium produced an array of chitinolytic enzymes representing significant components of the J4 strain secretome. The excreted active proteins were characterized by estimating the enzymatic activities of endochitinase, exochitinase, and N-acetylglucosaminidase induced by cultivation in medium M-10 with colloidalchitin. The enzyme activities produced by J4 strain cultivated in medium M-10 with glucose were significantly lower. The spectrum of extracellularly excreted proteins was separated by SDS-PAGE. The chitinase variability was confirmed on zymograms of renatured SDS-PAGE. The enzymes were visualized under ultraviolet light by using 4-methylumbelliferyl derivatives of N-acetyl-beta-d-glucosaminide, N,NA '-diacetyl-beta-d-chitobiose, or N,NA ',NEe-triacetyl-beta-d-chitotriose for beta-N-acety

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

<a href="/en/project/GA310%2F09%2F1407" target="_blank" >GA310/09/1407: Chitinolytic enzymes from Clostridium paraputrificum inhabiting the human gastrointestinal tract; structure and biomedical implications.</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Folia Microbiologica

ISSN

0015-5632

e-ISSN

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Volume of the periodical

57

Issue of the periodical within the volume

4

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

5

Pages from-to

335-339

UT code for WoS article

000305980400019

EID of the result in the Scopus database

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