Targeting adenovirus gene delivery to activated tumour-associated vasculature via endothelial selectins

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F11%3A00358495" target="_blank" >RIV/61389013:_____/11:00358495 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.jconrel.2010.10.011" target="_blank" >http://dx.doi.org/10.1016/j.jconrel.2010.10.011</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jconrel.2010.10.011" target="_blank" >10.1016/j.jconrel.2010.10.011</a>

Result language
angličtina
Original language name
Targeting adenovirus gene delivery to activated tumour-associated vasculature via endothelial selectins
Original language description
Adenovirus (Adluc) was coated with a reactive polymer based on poly[N-(2-hydroxypropyl)methacrylamide] to ablate normal infection pathways. Linkage of a monoclonal antibody against E-selectin demonstrated E-selectin-specific transduction of tumour necrosis factor-? (TNF-?)-activated endothelial cells. A two-component targeting system using protein G was developed. We report an enhancement in transduction of TNF-?-activated endothelium in vitro and ex vivo in a human umbilical vein cord model using the E-selectin antibody. Virus retargeted using a chimeric P-selectin Glycoprotein Ligand-1-Fc fusion (PSGL-1) protein showed better circulation kinetics and uptake into HepG2 xenografts following systemic administration in mice, with 36-fold higher genome copies, compared with non-modified virus. Immunohistochemistry of tumour sections from mice treated with PSGL-1-retargeted virus showed a co-localisation of luciferase with CD31 suggesting selective endothelial targeting.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EI - Biotechnology and bionics
OECD FORD branch
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Project
<a href="/en/project/1M0505" target="_blank" >1M0505: Center of targeted therapeutic drugs</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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