E-selectin is a viable route of infection for polymer-coated adenovirus retargeting in TNF-.alpha.-activated human umbilical vein endothelial cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F11%3A00362894" target="_blank" >RIV/61389013:_____/11:00362894 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.3109/1061186X.2010.547585" target="_blank" >http://dx.doi.org/10.3109/1061186X.2010.547585</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3109/1061186X.2010.547585" target="_blank" >10.3109/1061186X.2010.547585</a>
Alternative languages
Result language
angličtina
Original language name
E-selectin is a viable route of infection for polymer-coated adenovirus retargeting in TNF-.alpha.-activated human umbilical vein endothelial cells
Original language description
Retargeting of adenovirus via E-selectin as a viable pathway of infection in tumor necrosis factor-? (TNF-?)-activated human umbilical vein endothelial cells (HUVECs) was investigated. E1, E3-deleted Ad5 expressing cytomegalovirus immediate-early promoter-driven luciferase (Adluc) was coated with a reactive multivalent copolymer based on poly [N-(2-hydroxypropyl) methacrylamide] to generate pHPMA-adenovirus (pcAdluc). This was then retargeted by covalent attachment of a mouse antihuman E-selectin monoclonal antibody (MHES mAb). MHESpcAdluc was efficiently taken up into HUVECs, generating a high level of transduction in TNF-?-treated E-selectin positive cells but not in untreated receptor-negative cells. Our results suggest that E-selectin could be a valuable target for gene transfer strategies internalizing polymer-coated adenovirus particles through a viable receptor-mediated endocytosis pathway, generating adequate levels of transgene expression per virus genome copy.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CD - Macromolecular chemistry
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Drug Targeting
ISSN
1061-186X
e-ISSN
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Volume of the periodical
19
Issue of the periodical within the volume
8
Country of publishing house
GB - UNITED KINGDOM
Number of pages
11
Pages from-to
690-700
UT code for WoS article
000293743800012
EID of the result in the Scopus database
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