SAXS study of sterically stabilized lipid nanocarriers functionalized by DNA

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F12%3A00376686" target="_blank" >RIV/61389013:_____/12:00376686 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1088/1742-6596/351/1/012004" target="_blank" >http://dx.doi.org/10.1088/1742-6596/351/1/012004</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1088/1742-6596/351/1/012004" target="_blank" >10.1088/1742-6596/351/1/012004</a>

Result language
angličtina
Original language name
SAXS study of sterically stabilized lipid nanocarriers functionalized by DNA
Original language description
The structure of novel spontaneously self-assembled plasmid DNA/lipid complexes is investigated by means of small-angle X-ray scattering (SAXS) and Cryo-TEM imaging. Liquid crystalline (LC) hydrated lipid systems are prepared using the nonionic lipids monoolein and DOPE-PEG2000 and the cationic amphiphile CTAB. The employed plasmid DNA (pDNA) is encoding for the human protein brain-derived neurotrophic factor (BDNF). A coexistence of nanoparticulate objects with different LC inner organizations is established. A transition from bicontinuous membrane sponges, cubosome intermediates and unilamelar liposomes to multilamellar vesicles, functionalized by pDNA, is favoured upon binding and compaction of pBDNF onto the cationic PEGylated lipid nanocarriers. The obtained sterically stabilized nanovehicles, with confined supercoiled plasmid DNA (pBDNF), are important in the context of multicompartment lipid nanocarriers of interest for gene therapy of neurodegenerative diseases.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CD - Macromolecular chemistry
OECD FORD branch
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Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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