Polymer-modified gene delivery vectors retargeted with recombinant proteins

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F12%3A00381465" target="_blank" >RIV/61389013:_____/12:00381465 - isvavai.cz</a>
Result on the web
<a href="http://www.appleacademicpress.com/title.php?id=9781926895178" target="_blank" >http://www.appleacademicpress.com/title.php?id=9781926895178</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Polymer-modified gene delivery vectors retargeted with recombinant proteins
Original language description
We have prepared and characterized a multivalent reactive N-(2-hydroxypropyl)methacrylamide copolymer (PHPMA) bearing bungarotoxin-binding peptide (BTXbp). The polymer was used for covalent surface modification of adenoviral vectors containing luciferasereporter gene (Ad). The peptide BTXbp is known to have extremely high binding affinity to bungarotoxin (BTX) ?a protein strongly binding to acetylcholine receptors. A recombinant protein consisting of antiPSMA antibody scFv fragment and BTX binding site(BTX-scFv) was used for retargeting of the polymer-modified Ad to prostate-specific membrane antigen (PSMA) receptors. While the polymer-modified Ad exhibited approximately 100-fold lower infectivity than the naked virus (in terms of luciferase expression), the addition of BTX-scFv to the polymer-coated Ad led to about 10-fold restoration of luciferase expression in PSMA-positive LNCaP cells. No such increase of transduction activity was observed in PSMA-negative PC3 cells. We have show
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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