Diagnosis of Epstein-Barr virus infection in clinical serum samples by an SPR biosensor assay

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F14%3A00427702" target="_blank" >RIV/61389013:_____/14:00427702 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.bios.2013.12.011" target="_blank" >http://dx.doi.org/10.1016/j.bios.2013.12.011</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bios.2013.12.011" target="_blank" >10.1016/j.bios.2013.12.011</a>

Result language

angličtina

Original language name

Diagnosis of Epstein-Barr virus infection in clinical serum samples by an SPR biosensor assay

Original language description

Label-free affinity biosensors offer a promising platform for the development of a new generation of medical diagnostic technologies. Nevertheless, when such sensors are used in complex biological media, adsorption of non-targeted medium components prevents the specific detection of the analyte. In this work, we introduce for the first time a biosensor assay based on surface plasmon resonance (SPR) capable of diagnosing different stages of Epstein?Barr virus (EBV) infections in clinical serum samples. This was achieved by simultaneous detection of the antibodies against three different antigens present in the virus. To prevent the interference of the fouling from serum during the measurement, the SPR chips were coated by an antifouling layer of a polymer brush of poly[oligo(ethylene glycol) methacrylate] grown by surface-initiated atom transfer radical polymerization. The bioreceptors were then attached via hybridization of complementary oligonucleotides. This allowed the sensor surfac

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biosensors and Bioelectronics

ISSN

0956-5663

e-ISSN

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Volume of the periodical

55

Issue of the periodical within the volume

15 May

Country of publishing house

GB - UNITED KINGDOM

Number of pages

7

Pages from-to

278-284

UT code for WoS article

000332426100044

EID of the result in the Scopus database

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