Oxidative stress response in neural stem cells exposed to different superparamagnetic iron oxide nanoparticles

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F16%3A00458994" target="_blank" >RIV/61389013:_____/16:00458994 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.2147/IJN.S102730" target="_blank" >http://dx.doi.org/10.2147/IJN.S102730</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2147/IJN.S102730" target="_blank" >10.2147/IJN.S102730</a>

Result language

angličtina

Original language name

Oxidative stress response in neural stem cells exposed to different superparamagnetic iron oxide nanoparticles

Original language description

Biocompatibility, safety, and risk assessments of superparamagnetic iron oxide nanoparticles (SPIONs) are of the highest priority in researching their application in biomedicine. One improvement in the biological properties of SPIONs may be achieved by different functionalization and surface modifications. This study aims to investigate how a different surface functionalization of SPIONs – uncoated, coated with D-mannose, or coated with poly-L-lysine – affects biocompatibility. We sought to investigate murine neural stem cells (NSCs) as important model system for regenerative medicine. To reveal the possible mechanism of toxicity of SPIONs on NSCs, levels of reactive oxygen species, intracellular glutathione, mitochondrial membrane potential, cell-membrane potential, DNA damage, and activities of SOD and GPx were examined. Even in cases where reactive oxygen species levels were significantly lowered in NSCs exposed to SPIONs, we found depleted intracellular glutathione levels, altered activities of SOD and GPx, hyperpolarization of the mitochondrial membrane, dissipated cell-membrane potential, and increased DNA damage, irrespective of the surface coating applied for SPION stabilization. Although surface coating should prevent the toxic effects of SPIONs, our results showed that all of the tested SPION types affected the NSCs similarly, indicating that mitochondrial homeostasis is their major cellular target. Despite the claimed biomedical benefits of SPIONs, the refined determination of their effects on various cellular functions presented in this work highlights the need for further safety evaluations. This investigation helps to fill the knowledge gaps on the criteria that should be considered in evaluating the biocompatibility and safety of novel nanoparticles.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CD - Macromolecular chemistry

OECD FORD branch

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Project

<a href="/en/project/GC16-01128J" target="_blank" >GC16-01128J: Antioxidative magnetic nanoparticles based on natural antioxidants: Nanoparticle-cell interactions</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal of Nanomedicine

ISSN

1176-9114

e-ISSN

—

Volume of the periodical

11

Issue of the periodical within the volume

26 April

Country of publishing house

NZ - NEW ZEALAND

Number of pages

15

Pages from-to

1701-1715

UT code for WoS article

000374744800003

EID of the result in the Scopus database

2-s2.0-84964414204