Does surface coating of metallic nanoparticles modulate their interference with in vitro assays?
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F15%3A00446656" target="_blank" >RIV/61389013:_____/15:00446656 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1039/C5RA14100A" target="_blank" >http://dx.doi.org/10.1039/C5RA14100A</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1039/C5RA14100A" target="_blank" >10.1039/C5RA14100A</a>
Alternative languages
Result language
angličtina
Original language name
Does surface coating of metallic nanoparticles modulate their interference with in vitro assays?
Original language description
Screening programs for the evaluation of nanomaterial value and safety rely on in vitro tests. The exceptional physicochemical properties of metallic nanoparticles (NPs), such as large surface area and chemically active surface, may provoke their interference with in vitro methods and analytical techniques used for evaluation of biocompatibility or toxicity of NPs. This study aimed to determine if such interference could be predicted on the basis of the surface characteristics of metallic NPs by investigating the effect of different surface coatings of silver (AgNPs) and maghemite NPs (?-Fe2O3NPs) on common in vitro assays scoring two of the main cytotoxic endpoints: cell viability and oxidative stress response. We examined optical, adsorptive and chemically reactive types of NP interference with cell viability assays (MTT, MTS, and WST-8) and assays employing fluorescent dyes as markers for production of reactive oxygen species (DCFH-DA and DHE) or glutathione level (MBCl). Each type
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CD - Macromolecular chemistry
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
RSC Advances
ISSN
2046-2069
e-ISSN
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Volume of the periodical
5
Issue of the periodical within the volume
87
Country of publishing house
GB - UNITED KINGDOM
Number of pages
21
Pages from-to
70787-70807
UT code for WoS article
000360445500013
EID of the result in the Scopus database
2-s2.0-84940551027