Biocompatibility assessment of up-and down-converting nanoparticles: implications of interferences with in vitro assays

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F19%3A00497918" target="_blank" >RIV/61389013:_____/19:00497918 - isvavai.cz</a>

Result on the web

<a href="https://iopscience.iop.org/article/10.1088/2050-6120/aae9c8" target="_blank" >https://iopscience.iop.org/article/10.1088/2050-6120/aae9c8</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1088/2050-6120/aae9c8" target="_blank" >10.1088/2050-6120/aae9c8</a>

Result language

angličtina

Original language name

Biocompatibility assessment of up-and down-converting nanoparticles: implications of interferences with in vitro assays

Original language description

The safety assessment of nanoparticles (NPs) is crucial during their design and development for biomedicine. One of the prerequisite steps during this evaluation is in vitro testing that employs cell-based assays not always validated and well-adapted for NPs. Interferences with in vitro assays may arise due to the nano-related optical, oxidative, fluorescent, surface and catalytic properties of NPs. Thus, proper validation of each assay system has to be performed for each NP type. This study aimed to evaluate the applicability of the most common in vitro cytotoxicity assays for the safety assessment of up- and down-converting lanthanide-doped NPs. Conventional cell viability tests and fluorescence-based assays for oxidative stress response were selected to determine the biological effects of up- and down-converting NPs to human brain cells. Comparison with known silver and iron oxide NPs was made for verification purposes. Both the plate reader and flow cytometric measurements were examined. The obtained results indicated that both types of Ln-doped NPs interfered to a much lesser extent than metallic NPs. In addition, the great potential of both up- and down-converting NPs for biomedicine was manifested due to their biocompatibility and low toxicity.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10404 - Polymer science

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Methods and Applications in Fluorescence

ISSN

2050-6120

e-ISSN

—

Volume of the periodical

7

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

15

Pages from-to

1-15

UT code for WoS article

000517123400001

EID of the result in the Scopus database

2-s2.0-85056273391