Poly(amino acid)-based fibrous scaffolds modified with surface-pendant peptides for cartilage tissue engineering

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F17%3A00472812" target="_blank" >RIV/61389013:_____/17:00472812 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1002/term.1982" target="_blank" >http://dx.doi.org/10.1002/term.1982</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/term.1982" target="_blank" >10.1002/term.1982</a>

Result language
angličtina
Original language name
Poly(amino acid)-based fibrous scaffolds modified with surface-pendant peptides for cartilage tissue engineering
Original language description
In this study, fibrous scaffolds based on poly(gamma-benzyl-l-glutamate) (PBLG) were investigated in terms of the chondrogenic differentiation potential of human tooth germ stem cells (HTGSCs). Through the solution-assisted bonding of the fibres, fully connected scaffolds with pore sizes in the range 20–400 µm were prepared. Biomimetic modification of the PBLG scaffolds was achieved by a two-step reaction procedure: first, aminolysis of the PBLG fibres’ surface layers was performed, which resulted in an increase in the hydrophilicity of the fibrous scaffolds after the introduction of N5-hydroxyethyl-l-glutamine units. Second, modification with the short peptide sequence azidopentanoyl–GGGRGDSGGGY–NH2, using the 'click' reaction on the previously modified scaffold with 2-propynyl side-chains, was performed. Radio-assay of the 125I-labelled peptide was used to evaluate the RGD density in the fibrous scaffolds (which varied in the range 10–3–10 pm/cm2). All the PBLG scaffolds, especially with density 90 ± 20 fm/cm2 and 200 ± 100 fm/cm2 RGD, were found to be potentially suitable for growth and chondrogenic differentiation of HTGSCs.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10404 - Polymer science

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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