Chondrogenic potential of macroporous biodegradable cryogels based on synthetic poly(a-amino acids)
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F17%3APU127266" target="_blank" >RIV/00216305:26620/17:PU127266 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1039/c7sm02074k" target="_blank" >http://dx.doi.org/10.1039/c7sm02074k</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1039/c7sm02074k" target="_blank" >10.1039/c7sm02074k</a>
Alternative languages
Result language
angličtina
Original language name
Chondrogenic potential of macroporous biodegradable cryogels based on synthetic poly(a-amino acids)
Original language description
In this study, the potential of highly porous hydrogels based on biodegradable synthetic poly(a-amino acids) to support proliferation and chondrogenesis of human dental pulp stem cells (hDPSCs) was investigated. Covalently crosslinked gels with permanent pores were formed under cryogenic conditions by free-radical copolymerization of poly[N5-(2-hydroxyethyl)-L-glutamine-stat-N5-(2-methacryloyl-oxyethyl)- L-glutamine] (PHEG-MA) with 2-hydroxyethyl methacrylate (HEMA) and N-propargyl methacrylamide (PrMAAm) as minor co-monomers. PrMAAm provided alkyne groups for modifying the gels with cellsupporting moieties (RGDS peptides) by the azide–alkyne click’’-reaction. Two types of gels with different compressive moduli were prepared. Each type was modified with two different concentrations of RGDS peptide. X-ray computed nanotomography (nanoCT) was used to visualize and analyze the 3D-structure of the cryogels. It was shown that modifying the PHEG-MA cryogels within the range of RGDS concentrations examined here had a positive effect on the proliferation of hDPSCs. Immunofluorescence staining for collagen type 2 and aggrecan proved that there was differentiation of hDPSCs into chondrocytes.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
20501 - Materials engineering
Result continuities
Project
<a href="/en/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Soft Matter
ISSN
1744-683X
e-ISSN
1744-6848
Volume of the periodical
neuveden
Issue of the periodical within the volume
14
Country of publishing house
GB - UNITED KINGDOM
Number of pages
11
Pages from-to
228-238
UT code for WoS article
000419352600006
EID of the result in the Scopus database
2-s2.0-85040250837