Chondrogenic potential of macroporous biodegradable cryogels based on synthetic poly(.alpha.-amino acids)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F18%3A00483972" target="_blank" >RIV/61389013:_____/18:00483972 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1039/C7SM02074K" target="_blank" >http://dx.doi.org/10.1039/C7SM02074K</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/C7SM02074K" target="_blank" >10.1039/C7SM02074K</a>

Result language

angličtina

Original language name

Chondrogenic potential of macroporous biodegradable cryogels based on synthetic poly(.alpha.-amino acids)

Original language description

In this study, the potential of highly porous hydrogels based on biodegradable synthetic poly(α-amino acids) to support proliferation and chondrogenesis of human dental pulp stem cells (hDPSCs) was investigated. Covalently crosslinked gels with permanent pores were formed under cryogenic conditions by free-radical copolymerization of poly[N5-(2-hydroxyethyl)-L-glutamine-stat-N5-(2-methacryloyl-oxy-ethyl)-L-glutamine] (PHEG-MA) with 2-hydroxyethyl methacrylate (HEMA) and N-propargyl methacrylamide (PrMAAm) as minor co-monomers. PrMAAm provided alkyne groups for modifying the gels with cell-supporting moieties (RGDS peptides) by the azide–alkyne “click”-reaction. Two types of gels with different compressive moduli were prepared. Each type was modified with two different concentrations of RGDS peptide. X-ray computed nanotomography (nanoCT) was used to visualize and analyze the 3D-structure of the cryogels. It was shown that modifying the PHEG-MA cryogels within the range of RGDS concentrations examined here had a positive effect on the proliferation of hDPSCs. Immunofluorescence staining for collagen type 2 and aggrecan proved that there was differentiation of hDPSCs into chondrocytes.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10404 - Polymer science

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Soft Matter

ISSN

1744-683X

e-ISSN

—

Volume of the periodical

14

Issue of the periodical within the volume

2

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

228-238

UT code for WoS article

000419352600006

EID of the result in the Scopus database

2-s2.0-85040250837