Biological evaluation of surface-modified magnetic nanoparticles as a platform for colon cancer cell theranostics

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F18%3A00482869" target="_blank" >RIV/61389013:_____/18:00482869 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.colsurfb.2017.10.034" target="_blank" >http://dx.doi.org/10.1016/j.colsurfb.2017.10.034</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.colsurfb.2017.10.034" target="_blank" >10.1016/j.colsurfb.2017.10.034</a>

Result language

angličtina

Original language name

Biological evaluation of surface-modified magnetic nanoparticles as a platform for colon cancer cell theranostics

Original language description

Magnetic nanoparticles offer multiple possibilities for biomedical applications. Besides their physico-chemical properties, nanoparticle-cellular interactions are determinant for biological safety. In this work, magnetic nanoparticles were synthesized by one-shot precipitation or two-step reaction and coated with biocompatible polymers, such as poly(l-lysine) and poly(N,N-dimethylacrylamide-co-acrylic acid), and carbohydrates, like l-ascorbic acid, d-galactose, d-mannose, and sucrose. The resulting magnetic nanoparticles were characterized by dynamic light scattering, FT-Raman spectroscopy, transmission electron microscopy, SQUID magnetometry, and Mössbauer spectroscopy. Ability of the nanoparticles to be used in theranostic applications was also evaluated, showing that coating with biocompatible polymers increased the heating efficiency. Nanoparticles synthesized by one-shot precipitation were 50% larger (∼13 nm) than those obtained by a two-step reaction (∼8 nm). Magnetic nanoparticles at concentrations up to 500 μg mL−1 were non-cytotoxic to L929 fibroblasts. Particles synthesized by one-shot precipitation had little effect on viability, cell cycle and apoptosis of the three human colon cancer cell lines used: Caco-2, HT-29, and SW-480. At the same concentration (500 μg mL−1), magnetic particles prepared by a two-step reaction reduced colon cancer cell viability by 20%, affecting cell cycle and inducing cell apoptosis. Uptake of surface-coated magnetic nanoparticles by colon cancer cells was dependent on particle synthesis, surface coating and incubation time.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10404 - Polymer science

Project

<a href="/en/project/GC16-01128J" target="_blank" >GC16-01128J: Antioxidative magnetic nanoparticles based on natural antioxidants: Nanoparticle-cell interactions</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Colloids and Surfaces B-Biointerfaces

ISSN

0927-7765

e-ISSN

—

Volume of the periodical

161

Issue of the periodical within the volume

1 January

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

7

Pages from-to

35-41

UT code for WoS article

000423636100005

EID of the result in the Scopus database

2-s2.0-85032858139