Distribution of diffusion times determined by fluorescence (lifetime) correlation spectroscopy
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F18%3A00489135" target="_blank" >RIV/61389013:_____/18:00489135 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1021/acs.macromol.7b02158" target="_blank" >http://dx.doi.org/10.1021/acs.macromol.7b02158</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acs.macromol.7b02158" target="_blank" >10.1021/acs.macromol.7b02158</a>
Alternative languages
Result language
angličtina
Original language name
Distribution of diffusion times determined by fluorescence (lifetime) correlation spectroscopy
Original language description
Fluorescence correlation spectroscopy (FCS) provides most commonly values of diffusion coefficients of fluorescently labeled species in a solution, especially biopolymers. A newly developed procedure for the determination of diffusion coefficient distributions applicable to polydisperse polymers or nanoparticles, based on the well-known CONTIN algorithm, is described and tested on both simulated FCS correlation functions and real experimental data. Good resolution of bimodal distributions is observed, and it is quantitatively established how the resolution depends on the level of experimental noise. Effects of incorrect calibration of the focal volume on the obtained diffusion coefficient and its distribution are described for single-focus FCS. With rapid expansion of the FCS method from biology and biochemistry into the polymer field, and recently into many other disciplines including, e.g., environmental sciences, such a technique was very much needed.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10404 - Polymer science
Result continuities
Project
<a href="/en/project/LO1507" target="_blank" >LO1507: Polymers for Advanced Technologies and a Better Quality of Life</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Macromolecules
ISSN
0024-9297
e-ISSN
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Volume of the periodical
51
Issue of the periodical within the volume
8
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
2796-2804
UT code for WoS article
000431088700005
EID of the result in the Scopus database
2-s2.0-85045895401