Surface sticking and lateral diffusion of lipids in supported bilayers
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F06%3A00043204" target="_blank" >RIV/61388955:_____/06:00043204 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Surface sticking and lateral diffusion of lipids in supported bilayers
Original language description
The diffusion of fluorescently labeled lipids in supported bilayers is studied using two different methods: Z-scan fluorescence correlation spectroscopy (z-scan FCS) and two-focus fluorescence correlation spectroscopy (2f-FCS). It is found that the datacan be fitted consistently only when taking into account partial sticking of the labeled lipids to the supporting glass surface. A kinetic reaction-diffusion model is developed and applied to the data. We find a very slow sticking rate which, however, when neglected, leads to strongly varying estimates of the free diffusion coefficient. The study reveals a strong sensitivity of FCS on even slight binding/unbinding kinetics of the labeled molecules, which has significance for related diffusion measurements in cellular lipid membranes.
Czech name
Povrchové ulpívání a laterální difúze lipidů v podporovaných dvojvrstvách
Czech description
Difůze fluorescenčně značených lipidů byla sledována pomocí ?Z-scans fluorescnční korelační spektroskopie? a dvou ohniskové fluorescenční korelační spektroskopie. Z naměřených dat je patrné ulpívání molekul lipidu na povrchu. Tento proces je velmi pomalý, nicméně jeho zanedbání vede ke zkresleným hodnotám difůzního koeficientu.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CF - Physical chemistry and theoretical chemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA203%2F05%2F2308" target="_blank" >GA203/05/2308: Understanding, control, and preparation of self-assembling biomembrane systems at interfaces</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2006
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Langmuir
ISSN
0743-7463
e-ISSN
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Volume of the periodical
22
Issue of the periodical within the volume
22
Country of publishing house
US - UNITED STATES
Number of pages
6
Pages from-to
9339-9344
UT code for WoS article
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EID of the result in the Scopus database
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