Adsorption of lysozyme on pH-responsive PnBA-b-PAA polymeric nanoparticles: studies by stopped-flow SAXS and ITC
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F18%3A00490445" target="_blank" >RIV/61389013:_____/18:00490445 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/s00396-018-4329-4" target="_blank" >http://dx.doi.org/10.1007/s00396-018-4329-4</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00396-018-4329-4" target="_blank" >10.1007/s00396-018-4329-4</a>
Alternative languages
Result language
angličtina
Original language name
Adsorption of lysozyme on pH-responsive PnBA-b-PAA polymeric nanoparticles: studies by stopped-flow SAXS and ITC
Original language description
The interactions of poly(n-butyl acrylate)-b-poly(acrylic acid) (PnBA-b-PAA) block copolymer nanoparticles with lysozyme were examined by a stopped-flow technique consisting of small-angle X-ray scattering (SAXS) and isothermal titration calorimetry (ITC). Nanoparticles composed of PnBA-b-PAA block copolymer had a spherical shape and core-shell structure, which was confirmed by cryo-transmission electron microscopy (cryo-TEM) and SAXS. ITC revealed that lysozyme binds to the nanoparticle surface and that the strength of the binding depends on the ionic strength of the solution and the composition of the PnBA-b-PAA copolymers. Thermodynamic results unambiguously indicated that the interaction of lysozyme with the nanoparticle surface is a two-step process and entropy driven. Stopped-flow experiments proved that lysozyme penetration into the PAA corona occurs on a time scale of hundreds of milliseconds. Based on the fitting of SAXS curves, the number of micelles per volume increases with time, the core size remains nearly constant, and the thickness of the shell decreases. Such findings justify the model proposed in our previous papers.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10404 - Polymer science
Result continuities
Project
<a href="/en/project/LH15213" target="_blank" >LH15213: Behavior and shape of nanoparticles – anticancer drug carriers in real blood environment.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Colloid and Polymer Science
ISSN
0303-402X
e-ISSN
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Volume of the periodical
296
Issue of the periodical within the volume
7
Country of publishing house
DE - GERMANY
Number of pages
9
Pages from-to
1183-1191
UT code for WoS article
000435592300009
EID of the result in the Scopus database
2-s2.0-85047362735