Binding of HSA to macromolecular pHPMA based nanoparticles for drug delivery: an investigation using fluorescence methods
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F18%3A00491297" target="_blank" >RIV/61389013:_____/18:00491297 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1021/acs.langmuir.8b01015" target="_blank" >http://dx.doi.org/10.1021/acs.langmuir.8b01015</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acs.langmuir.8b01015" target="_blank" >10.1021/acs.langmuir.8b01015</a>
Alternative languages
Result language
angličtina
Original language name
Binding of HSA to macromolecular pHPMA based nanoparticles for drug delivery: an investigation using fluorescence methods
Original language description
Amphiphilic poly(N-(2-hydroxypropyl)methacrylamide) copolymers (pHPMA) bearing cholesterol side groups in phosphate buffer saline self-assemble into nanoparticles (NPs) which can be used as tumor-targeted drug carriers. It was previously shown by us that human serum albumin (HSA) interacts weakly with the NPs. However, the mechanism of this binding could not be resolved due to overlapping of signals from the complex system. Here, we use fluorescence labeling to distinguish the components and to characterize the binding: On the one hand, a fluorescent dye was attached to pHPMA, so that the diffusion behavior of the NPs could be studied in the presence of HSA using fluorescence lifetime correlation spectroscopy. On the other hand, quenching of the intrinsic fluorescence of HSA revealed the origin of the binding, which is mainly the complexation between HSA and cholesterol side groups. Furthermore, a binding constant was obtained.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10404 - Polymer science
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Langmuir
ISSN
0743-7463
e-ISSN
—
Volume of the periodical
34
Issue of the periodical within the volume
27
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
7998-8006
UT code for WoS article
000438653800006
EID of the result in the Scopus database
2-s2.0-85049753519