Antibody-pHPMA functionalised fluorescent silica nanoparticles for colorectal carcinoma targeting

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F18%3A00494583" target="_blank" >RIV/61389013:_____/18:00494583 - isvavai.cz</a>

Alternative codes found

RIV/61388971:_____/18:00494583 RIV/68378050:_____/18:00494583 RIV/60461373:22340/18:43916255

Result on the web

<a href="http://dx.doi.org/10.1039/c8ra03487g" target="_blank" >http://dx.doi.org/10.1039/c8ra03487g</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/c8ra03487g" target="_blank" >10.1039/c8ra03487g</a>

Result language

angličtina

Original language name

Antibody-pHPMA functionalised fluorescent silica nanoparticles for colorectal carcinoma targeting

Original language description

The systemic application of highly potent drugs such as cytostatics poses the risks of side effects, which could be reduced by using a carrier system able to specifically deliver the encapsulated drug to the target tissue. Essential components of a nanoparticle-based drug delivery system include the drug carrier itself, a targeting moiety, and a surface coating that minimizes recognition by the immune system. The present work reports on the preparation, in vitro characterization and in vivo testing of a new delivery system consisting of fluorescent silica nanoparticles functionalised with a non-immunogenic stealth polymer poly(N-(2-hydroxypropyl)methacrylamide) (pHPMA) and a monoclonal antibody IgG M75 that specifically binds to Carbonic Anhydrase IX (CA IX). CA IX is a promising therapeutic target, as it is a hallmark of several hypoxic tumours including colorectal carcinoma. Uniquely in this work, the monoclonal antibody was covalently coupled to the surface of fluorescently labelled silica nanoparticles via a multivalent amino-reactive co-polymer rather than a traditional bivalent linker. The pHPMA-M75 functionalised SiO2 nanoparticles exhibited excellent colloidal stability in physiological media. Their in vitro characterisation by flow cytometry proved a highly specific interaction with colorectal carcinoma cells HT-29. In vivo study on athymic NU/NU nude mice revealed that the SiO2-pHPMA-M75 nanoparticles are capable of circulating in the blood after intravenous administration and accumulate in the tumour at tenfold higher concentration than nanoparticles without specific targeting, with a considerably longer retention time. Additionally, it was found that by reducing the dose administered in vivo, the selectivity of the nanoparticle biodistribution could be further enhanced in favour of the tumour.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10404 - Polymer science

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

RSC Advances

ISSN

2046-2069

e-ISSN

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Volume of the periodical

8

Issue of the periodical within the volume

39

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

21679-21689

UT code for WoS article

000436031800002

EID of the result in the Scopus database

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