Optimalization of deoxyribonucleic acid extraction using various types of magnetic particles

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F19%3A00504208" target="_blank" >RIV/61389013:_____/19:00504208 - isvavai.cz</a>

Result on the web

<a href="https://link.springer.com/article/10.1007%2Fs11696-018-00675-9" target="_blank" >https://link.springer.com/article/10.1007%2Fs11696-018-00675-9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11696-018-00675-9" target="_blank" >10.1007/s11696-018-00675-9</a>

Result language

angličtina

Original language name

Optimalization of deoxyribonucleic acid extraction using various types of magnetic particles

Original language description

Isolation of deoxyribonucleic acid is an important step in the molecular diagnostics of microorganisms. A high quality of isolated deoxyribonucleic acid is necessary for deoxyribonucleic acid amplification by the polymerase chain reaction. The conventional deoxyribonucleic acid isolation using phenol–chloroform extraction and deoxyribonucleic acid precipitation in ethanol is time-consuming and requires the use of toxic phenol. Magnetic separation techniques using magnetic solid particles are one of modern methods to speed up the nucleic acids isolation. The aim of this work was to use two different types of magnetic particles for solid-phase deoxyribonucleic acid extraction. The amounts of deoxyribonucleic acid in separation mixtures were measured using ultraviolet spectrophotometry. The first experimental conditions were tested on chicken erythrocytes deoxyribonucleic acid. Phosphate buffer (pH 7, 7.6 and 8) was used for the adsorption of deoxyribonucleic acid on magnetic particles. Tested values of pH have no effects on deoxyribonucleic acid adsorption. It was shown that approximately almost one half of deoxyribonucleic acid was adsorbed to the particles. A number of different elution conditions (temperature, time and pH value of elution buffer) were investigated to determine their effect on elution efficiency. It was shown that only a small fraction of the bound DNA was released at 22 °C, while the release was more effective as the temperature was increased also the amount of elution deoxyribonucleic acid was more effective when time was increased. The higher amounts of deoxyribonucleic acid were eluted with TE buffer pH 9.0. Second, bacterial deoxyribonucleic acid was tested. This deoxyribonucleic acid eluted from the particles was in polymerase chain reaction ready quality.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10404 - Polymer science

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chemical Papers

ISSN

2585-7290

e-ISSN

—

Volume of the periodical

73

Issue of the periodical within the volume

5

Country of publishing house

SK - SLOVAKIA

Number of pages

9

Pages from-to

1247-1255

UT code for WoS article

000463985400020

EID of the result in the Scopus database

2-s2.0-85064202551