Singlet oxygen phosphorescence detection in vivo identifies PDT-induced anoxia in solid tumors

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F19%3A00505493" target="_blank" >RIV/61389013:_____/19:00505493 - isvavai.cz</a>

Result on the web

<a href="https://pubs.rsc.org/en/content/articlelanding/2019/PP/C8PP00570B#!divAbstract" target="_blank" >https://pubs.rsc.org/en/content/articlelanding/2019/PP/C8PP00570B#!divAbstract</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/C8PP00570B" target="_blank" >10.1039/C8PP00570B</a>

Result language

angličtina

Original language name

Singlet oxygen phosphorescence detection in vivo identifies PDT-induced anoxia in solid tumors

Original language description

Real-time surveillance of photodynamic therapy (PDT) has been desired by the research community for a long time. The impact of the treatment is encoded in the phosphorescence kinetics of its main mediator: singlet oxygen. We report successful in vivo measurements of these weak kinetics through the skin of living mice after systemic drug application. Using special high transmission optics centered around 1200, 1270 and 1340 nm, singlet oxygen phosphorescence can be clearly discriminated from other signals. N-(2-Hydroxypropyl)methacrylamide copolymers conjugated with pyropheophorbide-a exhibit highly selective accumulation in tumors. Signals of this drug in tumors were compared to those in normal tissue. In both places, the major part of the signal could be identified as arising from drug still circulating in the bloodstream. Despite high concentrations of extravasated drug in the tumors due to the EPR effect, nearly no signal could be detected from these photosensitizers in vivo, contradicting in vitro experiments. We propose that the reason for this discrepancy is oxygen depletion in tumor tissue in vivo, even at moderate (at PDT scale) illumination intensities, soon after the start of the illumination. These results underline the importance of singlet oxygen surveillance during PDT treatment.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10404 - Polymer science

Project

<a href="/en/project/NV16-28594A" target="_blank" >NV16-28594A: Advanced method for fluorescence-guided endoscopic surgery</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Photochemical & Photobiological Sciences

ISSN

1474-905X

e-ISSN

—

Volume of the periodical

18

Issue of the periodical within the volume

6

Country of publishing house

FR - FRANCE

Number of pages

11

Pages from-to

1304-1314

UT code for WoS article

000471136600029

EID of the result in the Scopus database

2-s2.0-85067253787