Singlet oxygen in vivo: it is all about intensity
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F22%3A00557755" target="_blank" >RIV/61389013:_____/22:00557755 - isvavai.cz</a>
Result on the web
<a href="https://www.mdpi.com/2075-4426/12/6/891" target="_blank" >https://www.mdpi.com/2075-4426/12/6/891</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/jpm12060891" target="_blank" >10.3390/jpm12060891</a>
Alternative languages
Result language
angličtina
Original language name
Singlet oxygen in vivo: it is all about intensity
Original language description
The presented work addresses the influence of illumination intensity on the amount and locations of singlet oxygen generation in tumor tissue. We used time-resolved optical detection at the typical emission wavelength around 1270 nm and at 1200 nm where there is no singlet oxygen phosphorescence to determine the phosphorescence kinetics. The discussed data comprise in vivo measurements in tumor-laden HET-CAM and mice. The results show that illumination that is too intense is a major issue, affecting many PDT treatments and all singlet oxygen measurements in vivo so far. In such cases, photosensitization and oxygen consumption exceed oxygen supply, limiting singlet oxygen generation to the blood vessels and walls, while photosensitizers in the surrounding tissue will likely not participate. Being a limitation for the treatment, on one hand, on the other, this finding offers a new method for tumor diagnosis when using photosensitizers exploiting the EPR effect. In contrast to high-intensity PDT, some papers reported successful treatment with nanoparticular drugs using much lower illumination intensity. The question of whether, with such illumination, singlet oxygen is indeed generated in areas apart from vessels and walls, is addressed by numerical analysis. In addition, we discuss how to perform measurements at such low intensities.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10404 - Polymer science
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Personalized Medicine
ISSN
2075-4426
e-ISSN
2075-4426
Volume of the periodical
12
Issue of the periodical within the volume
6
Country of publishing house
CH - SWITZERLAND
Number of pages
11
Pages from-to
891
UT code for WoS article
000815913600001
EID of the result in the Scopus database
2-s2.0-85131434537