Bioengineering a pre-vascularized pouch for subsequent islet transplantation using VEGF-loaded polylactide capsules
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F20%3A00520757" target="_blank" >RIV/61389013:_____/20:00520757 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11110/20:10402505 RIV/00216208:11120/20:43919202 RIV/00064173:_____/20:N0000126 RIV/00023001:_____/20:00079155
Result on the web
<a href="https://pubs.rsc.org/en/content/articlelanding/2020/BM/C9BM01280J#!divAbstract" target="_blank" >https://pubs.rsc.org/en/content/articlelanding/2020/BM/C9BM01280J#!divAbstract</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1039/C9BM01280J" target="_blank" >10.1039/C9BM01280J</a>
Alternative languages
Result language
angličtina
Original language name
Bioengineering a pre-vascularized pouch for subsequent islet transplantation using VEGF-loaded polylactide capsules
Original language description
he effectiveness of cell transplantation can be improved by optimization of the transplantation site. For some types of cells that form highly oxygen-demanding tissue, e.g., pancreatic islets, a successful engraftment depends on immediate and sufficient blood supply. This critical point can be avoided when cells are transplanted into a bioengineered pre-vascularized cavity which can be formed using a polymer scaffold. In our study, we tested surface-modified poly(lactide-co-caprolactone) (PLCL) capsular scaffolds containing the pro-angiogenic factor VEGF. After each modification step (i.e., amination and heparinization), the surface properties and morphology of scaffolds were characterized by ATR-FTIR and XPS spectroscopy, and by SEM and AFM. All modifications preserved the gross capsule morphology and maintained the open pore structure. Optimized aminolysis conditions decreased the Mw of PLCL only up to 10% while generating a sufficient number of NH2 groups required for the covalent immobilization of heparin. The heparin layer served as a VEGF reservoir with an in vitro VEGF release for at least four weeks. In vivo studies revealed that to obtain highly vascularized PLCL capsules (a) the optimal VEGF dose for the capsule was 50 μg and (b) the implantation time was four weeks when implanted into the greater omentum of Lewis rats. Dense fibrous tissue accompanied by vessels completely infiltrated the scaffold and created sparse granulation tissue within the internal cavity of the capsule. The prepared pre-vascularized pouch enabled the islet graft survival and functioning for at least 50 days after islet transplantation. The proposed construct can be used to create a reliable pre-vascularized pouch for cell transplantation.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10404 - Polymer science
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biomaterials Science
ISSN
2047-4830
e-ISSN
—
Volume of the periodical
8
Issue of the periodical within the volume
2
Country of publishing house
GB - UNITED KINGDOM
Number of pages
17
Pages from-to
631-647
UT code for WoS article
000509549500007
EID of the result in the Scopus database
2-s2.0-85078378760