Spinal parenchymal occupation by neural stem cells after subpial delivery in adult immunodeficient rats

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F20%3A00523980" target="_blank" >RIV/61389013:_____/20:00523980 - isvavai.cz</a>

Alternative codes found

RIV/67985904:_____/20:00523980

Result on the web

<a href="https://stemcellsjournals.onlinelibrary.wiley.com/doi/full/10.1002/sctm.19-0156" target="_blank" >https://stemcellsjournals.onlinelibrary.wiley.com/doi/full/10.1002/sctm.19-0156</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/sctm.19-0156" target="_blank" >10.1002/sctm.19-0156</a>

Result language

angličtina

Original language name

Spinal parenchymal occupation by neural stem cells after subpial delivery in adult immunodeficient rats

Original language description

Neural precursor cells (NSCs) hold great potential to treat a variety of neurodegenerative diseases and injuries to the spinal cord. However, current delivery techniques require an invasive approach in which an injection needle is advanced into the spinal parenchyma to deliver cells of interest. As such, this approach is associated with an inherent risk of spinal injury, as well as a limited delivery of cells into multiple spinal segments. Here, we characterize the use of a novel cell delivery technique that employs single bolus cell injections into the spinal subpial space. In immunodeficient rats, two subpial injections of human NSCs were performed in the cervical and lumbar spinal cord, respectively. The survival, distribution, and phenotype of transplanted cells were assessed 6-8 months after injection. Immunofluorescence staining and mRNA sequencing analysis demonstrated a near-complete occupation of the spinal cord by injected cells, in which transplanted human NSCs (hNSCs) preferentially acquired glial phenotypes, expressing oligodendrocyte (Olig2, APC) or astrocyte (GFAP) markers. In the outermost layer of the spinal cord, injected hNSCs differentiated into glia limitans-forming astrocytes and expressed human-specific superoxide dismutase and laminin. All animals showed normal neurological function for the duration of the analysis. These data show that the subpial cell delivery technique is highly effective in populating the entire spinal cord with injected NSCs, and has a potential for clinical use in cell replacement therapies for the treatment of ALS, multiple sclerosis, or spinal cord injury.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10404 - Polymer science

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Stem Cells Translational Medicine

ISSN

2157-6580

e-ISSN

—

Volume of the periodical

9

Issue of the periodical within the volume

2

Country of publishing house

US - UNITED STATES

Number of pages

12

Pages from-to

177-188

UT code for WoS article

000500683300001

EID of the result in the Scopus database

2-s2.0-85076210651