Selective potentiometric detection of reactive oxygen species (ROS) in biologically relevant concentrations by a modified metalized polyporphyrine sensing layer coated with nonbiofouling poly(2-alkyl-2oxazoline)s

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F22%3A00556452" target="_blank" >RIV/61389013:_____/22:00556452 - isvavai.cz</a>

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S0925400522004695?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0925400522004695?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.snb.2022.131827" target="_blank" >10.1016/j.snb.2022.131827</a>

Result language

angličtina

Original language name

Selective potentiometric detection of reactive oxygen species (ROS) in biologically relevant concentrations by a modified metalized polyporphyrine sensing layer coated with nonbiofouling poly(2-alkyl-2oxazoline)s

Original language description

We built a robust selective potentiometric sensor of reactive oxygen species (ROS) as a thin layer applied on the surface of smart implants, which enables the detection and localization of inflammation at an early stage. The sensor consists of a conductive polymer layer based on polythiophene with an incorporated porphyrin-metal complex that potentiometrically detects the presence of ROS. This sensor is covalently coated with a nonbiofouling layer of poly(2-methyl-2-oxazoline), which works as a biocompatibilizer but mainly prevents the sorption of proteins and other biomacromolecules naturally occurring in organisms, which could interfere with the ROS signal. We have shown that our potentiometric sensor shows a rapid response to hydrogen peroxide, does not experience interference with bovine serum albumin as a model serum protein when sensing ROS, is able to fully reversibly detect ROS with a linear response within a very wide range of biologically relevant concentrations and, most importantly, is able to distinguish between hydrogen peroxide and hypochlorite. We also performed a head-to-head comparison of two positional isomers of thienylated porphyrine for sensor applications (2TTP and 3TTP, with 3TTP shown to be more appropriate) and four different coordinated metals (Cu, Fe, Co and Mn, with Cu and especially Fe shown to be the most appropriate).

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10404 - Polymer science

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Sensors and Actuators B - Chemical

ISSN

0925-4005

e-ISSN

0925-4005

Volume of the periodical

363

Issue of the periodical within the volume

15 July

Country of publishing house

CH - SWITZERLAND

Number of pages

12

Pages from-to

131827

UT code for WoS article

000806144200005

EID of the result in the Scopus database

2-s2.0-85127821322