Tailoring butyl methacrylate/methacrylic acid copolymers for the solubilization of membrane proteins: the influence of composition and molecular weight
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F22%3A00562629" target="_blank" >RIV/61389013:_____/22:00562629 - isvavai.cz</a>
Alternative codes found
RIV/86652036:_____/22:00562629 RIV/68378050:_____/22:00562629 RIV/00216224:14740/22:00128767
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/10.1002/mabi.202200284" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1002/mabi.202200284</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/mabi.202200284" target="_blank" >10.1002/mabi.202200284</a>
Alternative languages
Result language
angličtina
Original language name
Tailoring butyl methacrylate/methacrylic acid copolymers for the solubilization of membrane proteins: the influence of composition and molecular weight
Original language description
Low-molecular weight (MW) amphiphilic copolymers have been recently introduced as a powerful tool for the detergent-free isolation of cell membrane proteins. Herein, a screening approach is used to identify a new copolymer type for this application. Via a two-step ATRP/acidolysis procedure, a 3 × 3 matrix of well-defined poly[(butyl methacrylate)-co-(methacrylic acid)] copolymers (denoted BMAA) differing in their MW and ratio of hydrophobic (BMA) and hydrophilic (MAA) units is prepared. Subsequently, using the biologically relevant model (T-cell line Jurkat), two compositions of BMAA copolymers are identified that solubilize cell membranes to an extent comparable to the industry standard, styrene-maleic acid copolymer (SMA), while avoiding the potentially problematic phenyl groups. Surprisingly, while only the lowest-MW variant of the BMA/MAA 2:1 composition is effective, all the copolymers of the BMA/MAA 1:1 composition are found to solubilize the model membranes, including the high-MW variant (MW of 14 000). Importantly, the density gradient ultracentrifugation/sodium dodecyl sulfate-polyacrylamide gel electrophoresis/Western blotting experiments reveal that the BMA/MAA 1:1 copolymers disintegrate the Jurkat membranes differently than SMA, as demonstrated by the different distribution patterns of two tested membrane protein markers. This makes the BMAA copolymers a useful tool for studies on membrane microdomains differing in their composition and resistance to membrane-disintegrating polymers.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10404 - Polymer science
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Macromolecular Bioscience
ISSN
1616-5187
e-ISSN
1616-5195
Volume of the periodical
22
Issue of the periodical within the volume
10
Country of publishing house
DE - GERMANY
Number of pages
7
Pages from-to
2200284
UT code for WoS article
000842344300001
EID of the result in the Scopus database
2-s2.0-85136506500