Proteinase K/retinoic acid-loaded cationic liposomes as multifunctional anti-acne therapy to disorganize biofilm and regulate keratinocyte proliferation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F23%3A00574148" target="_blank" >RIV/61389013:_____/23:00574148 - isvavai.cz</a>
Result on the web
<a href="https://www.dovepress.com/proteinase-kretinoic-acid-loaded-cationic-liposomes-as-multifunctional-peer-reviewed-fulltext-article-IJN" target="_blank" >https://www.dovepress.com/proteinase-kretinoic-acid-loaded-cationic-liposomes-as-multifunctional-peer-reviewed-fulltext-article-IJN</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2147/IJN.S416966" target="_blank" >10.2147/IJN.S416966</a>
Alternative languages
Result language
angličtina
Original language name
Proteinase K/retinoic acid-loaded cationic liposomes as multifunctional anti-acne therapy to disorganize biofilm and regulate keratinocyte proliferation
Original language description
Simultaneous anti-Cutibacterium acnes and anti-inflammatory actions are highly beneficial in treating acne vulgaris. In this study, we present novel anti-acne nanovesicles based on liposomes loaded with proteinase K (PK), retinoic acid (RA), and soyaethyl morpholinium ethosulfate (SME) to achieve an effective and safe treatment. This study examined in vitro planktonic and biofilm C. acnes elimination, as well as the keratinocyte proliferation suppression by liposomes. The multifunctional liposomes for treating C. acnes in mice were also evaluated. We acquired multifunctional liposomes with a size of 71 nm and zeta potential of 31 mV. The antimicrobial activity of SME was enhanced after liposomal encapsulation according to the reduction of minimum bactericidal concentration (MBC) by 6-fold. The multifunctional liposomes exhibited a synergistically inhibitory effect on biofilm C. acnes colonization compared with the liposomes containing PK or those containing SME individually. The adhesive bacterial colony in the microplate was lessened by 62% after multifunctional liposome intervention. All liposomal formulations tested here demonstrated no cytotoxicity against the normal keratinocytes but inhibited C. acnes-stimulated cell hyperproliferation. The in vitro scratch assay indicated that the liposomal RA—but not free RA—restrained keratinocyte migration. The animal study showed that free RA combined with SME and multifunctional nanovesicles had a similar effect on diminishing C. acnes colonies in the skin. On the other hand, liposomes exhibited superior performance in recovering the impaired skin barrier function than the free control. We also found that RA-loaded nanovesicles had greater skin tolerability than free RA. The cationic liposomes containing dual PK and RA represented a potential treatment to arrest bacterial infection and associated inflammation in acne.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10404 - Polymer science
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Nanomedicine
ISSN
1178-2013
e-ISSN
1178-2013
Volume of the periodical
18
Issue of the periodical within the volume
17 July
Country of publishing house
NZ - NEW ZEALAND
Number of pages
18
Pages from-to
3879-3896
UT code for WoS article
001031691600001
EID of the result in the Scopus database
2-s2.0-85165551146